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Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol.

Chabloz A, Schaefer JV, Kozieradzki I, Cronin SJF, Strebinger D, Macaluso F, Wald J, Rabbitts TH, Pluckthun A, Marlovits TC, Penninger JM
Commun Biol. 2020 Jul 3;3(1):342. doi: 10.1038/s42003-020-1072-4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
153322pCASP-SptP167-3G124-HilATriggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose
153323pCASP-SptP120-3G124-HilATriggers secretion of SptP120 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose
153324pCASP-3G124-HilAShows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vector
153325pCASP-SptP120-3m160-HilATriggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose
153326pCASP-SptP120-E3_5-HilATriggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinose
153327pCASP-SptP120-NS1-HilATriggers secretion of SptP120 fused to the NS1 monobody from Salmonella upon induction with arabinose
153328pCASP-SptP120-K27-HilATriggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose
153329pCASP-SptP120-K55-HilATriggers secretion of SptP120 fused to the K55 DARPin from Salmonella upon induction with arabinose
153330pCASP-SptP120-TD-HilATriggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose
153331pCASP-SptP92-TD-HilATriggers secretion of SptP92 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose
153332pCASP-SptP35-TD-HilATriggers expression of SptP35 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose
153333pCASP-HilA empty vectorTriggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed
153335Lenti-mCherry-WT-KRAS-IRES-BlastLentiviral vector plasmid for integration and constitutive expression of mCherry fused to wild-type KRAS in mammalian cells
153336Lenti-mCherry-G12V-KRAS-IRES-BlastLentiviral vector plasmid for integration and constitutive expression of mCherry fused to oncogenic G12V-KRAS in mammalian cells
154009Lenti-AcGFP-Sec61-IRES-BlastLentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)

Antibodies from Article