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pcDNA3-FLAG-KD LKB1

Plasmid

#8591

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K78I: lysine 78 mutated to isoleucine; kinase dead

Promoter

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

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Rtn3LΔ6LIR-mNeonGreen

Plasmid

#169036

Purpose

Express human Rtn3L with 6x LC3-interacting regions mutated in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rtn3L (RTN3 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Changed following LC3-interacting regions of Rtn3…

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-MYC

Plasmid

#130695

Purpose

Lentiviral plasmids encoding human MYC with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-FLAG-KD LKB1

Plasmid

#8593

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K78I: lysine 78 mutated to isoleucine; kinase dead

Promoter

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

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Rtn4a-mNeonGreen

Plasmid

#169032

Purpose

Express human Rtn4a

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rtn4a (RTN4 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2

Plasmid

#163403

Purpose

Expresses BMPR-2 in mammalican cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Limk1

Plasmid

#163414

Purpose

Expresses LIMK1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Limk1 (Limk1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1A-BRD3R

Plasmid

#130696

Purpose

Lentiviral plasmids encoding human BRD3R

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

Human BRD3R (BRD3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-cofilin S3A

Plasmid

#163604

Purpose

Expresses constitutively active cofilin in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

cofilin1 (Cfl1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

S3A substitution to make cofilin constitutively a…

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Bmp4

Plasmid

#163588

Purpose

Expresses BMP-4 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp4 (Bmp4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pYES1L-URA

Plasmid

#84301

Purpose

E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

Depositor

George Church

Article

Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-MCPIP1 (1-599)

Plasmid

#127461

Purpose

Expressing Flag-tagged human MCPIP1 full lengh protein in mammalian cells

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJH4687

Plasmid

#200783

Purpose

Pnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorin

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ins-22 (ins-22 Nematode)

Use

Tags

pHiuorin

Expression

Worm

Mutation

Promoter

Pnpr-4

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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p2xFLAGhYAP1-WW mutant

Plasmid

#17792

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 to Alanine; Proline 202 to Alanine

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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tetO-NKX2.5

Plasmid

#46029

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

NKX2.5 (NKX2-5 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

tetO

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp6

Plasmid

#163590

Purpose

Expresses BMP-6 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp6 (Bmp6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Cerulean-2xFv-hRIPK3

Plasmid

#106079

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

Cerulean-2xFv-hRIPK3 (RIPK3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only
BII-TOP-tdT

Plasmid

#133395

Purpose

Piggybac vector for canonical Wnt signaling reporter

Depositor

Blair Madison

Article

Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

Insert

tdTomato-NLS

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

SuperTOPFlash

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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XE118 CAMKII-T286D-CS2+

Plasmid

#16736

Depositor

Randall Moon

Article

Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

Insert

CAMKII-T286D (Camk2a Rat)

Use

Xenopus expression

Tags

Expression

Mutation

threonine 286 mutated to aspartate

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pCS2 GFP-GSK3mut-MAPK

Plasmid

#29690

Purpose

GFP reporter protein fused to three mutated GSK3 phosphorylation sites

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK

Use

Tags

Flag and Flag-EGFP

Expression

Mammalian

Mutation

recognition sites only, and E3 ligase site, GSK3 …

Promoter

SP6

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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