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PurposeLentiviral plasmids encoding human MYC with co-expressoin of GFP mediated by P2A.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 130695 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVH-EF1a-GFP-P2A
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehuman MYC
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SpeciesH. sapiens (human)
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Insert Size (bp)1320
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Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
We extensively modified the plasmid of pLVX-AcGFP-C1 (Clontech, 632155) by replacing CMV promoter with EF1a promoter, adding P2A to mediate co-expression of the gene of interest, and removal of PGK promoter and puromycin resistant gene to make a lentiviral backbone vector of pLVH-EF1a-P2A.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVH-EF1a-GFP-P2A-MYC was a gift from Kejin Hu (Addgene plasmid # 130695 ; http://n2t.net/addgene:130695 ; RRID:Addgene_130695) -
For your References section:
The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Shao Z, Zhang R, Khodadadi-Jamayran A, Chen B, Crowley MR, Festok MA, Crossman DK, Townes TM, Hu K. Nat Commun. 2016 Mar 7;7:10869. doi: 10.1038/ncomms10869. 10.1038/ncomms10869 PubMed 26947130