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Plasmid#154141PurposeExpresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.DepositorInsertscFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
UseTagsHA and sfGFPExpressionMammalianMutationR887EPromoterSFFVAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR iSH-YFP-PIF
Plasmid#50841PurposeOptogenetic PI3K activation system; cytoplasmic componentDepositorInsertsp85 iSH2
PIF6 1-100
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F271-OKT3
Plasmid#190682PurposeEncodes an EGFRvIII-CD3 BITE protein (F271 clone)DepositorInsertAnti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
PAmCherry1-N1-Tubbyc
Plasmid#202677PurposePhotoactivatable lipid biosensorDepositorInsertMus musculus Tub (243-505): PAmCherry1 (Tub Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV Cbx1-V5
Plasmid#175106PurposeLentiviral expression of mouse Cbx1-V5DepositorInsertCbx1 (Cbx1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-3XFLAG-Dvl2
Plasmid#123587DepositorInsertdishevelled segment polarity protein 2 (Dvl2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-H3.3-N-14
Plasmid#55302PurposeLocalization: Nucleus/Histones, Excitation: 399, Emission: 456DepositorInsertH3.3 (H3f3b Mouse)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Numb-mCherry
Plasmid#174193PurposeExpresses Numb in mammalian cellsDepositorInsertNumb (Numb Mouse)
UseTagsmCherryExpressionMammalianMutationContains Numb aa 8-593PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGFP-mHP1gamma
Plasmid#181902PurposeFluorescently tagged HP1gammaDepositorInsertmHP1gamma (Cbx3 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ms-Sox7
Plasmid#50780Purposeretroviral expression of mouse Sox7DepositorInsertSox7 (Sox7 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
ANO6-Plvx-mCherry-c1
Plasmid#62554PurposeFull length mouse ANO6 tagged with mCherry on the C-terminusDepositorInsertmouse ANO6 (Ano6 Mouse)
UseLentiviralTagsmCherryExpressionMutationPromoterCMVAvailable sinceMarch 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tet1-dCas9
Plasmid#136650PurposeTet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertTet1 (Tet1 Mouse)
UseCRISPRTags3XFLag-NLS-Tet1-dCas9-NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2-mDux
Plasmid#138320PurposeDox-inducible codon-optimized mouse DuxDepositorInsertDux (Dux Mouse)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterTREAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-KLC3-N-18
Plasmid#62760PurposeLocalization: Kinesin Light Chain , N1 Cloning Vector, Excitation:487, Emission:509DepositorInsertKLC3 (Klc3 Mouse)
UseTagsmEmeraldExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1 Calreticulin
Plasmid#191940PurposeExpression of calreticulinDepositorInsertCalreticulin (Calr Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF5-FRT-TagRFP-T-IFT88
Plasmid#61684PurposeMammalian expression of IFT88 fused with N terminal TagRFP.T tagDepositorInsertIFT88 (Ift88 Mouse)
UseTagsTagRFP.TExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)
Plasmid#101299PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-698 and 698-906 of alpha-C…PromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17
Plasmid#193298Purposeepisomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCsDepositorUseTagsExpressionMammalianMutationSox2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby
Plasmid#205280PurposeSynaptic activity can be imaged with Synaptophysin GCaMP, while simultaneously, a red fluorescence structural marker can be employed to provide a visual reference for the cellular architecture.DepositorInsertGCaMP8s
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-NLRP3 711-1034
Plasmid#75141PurposeExpresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (Nlrp3 Mouse)
UseTagsFlagExpressionMammalianMutationfragment encoding amino acids 711-1034PromoterCMVAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only