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Plasmid#28105DepositorInsertWIP1 (PPM1D Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMV-SNAP-hCB1_IRES_EGFP
Plasmid#223505PurposeExpression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection markerDepositorInsertSNAP-tag-CNR1 and EGFP (CNR1 Human, Synthetic)
UseTagsSNAP-tagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
nNOS/NOS1 scFv [L121/133]
Plasmid#206759PurposeMammalian Expression of nNOS/NOS1 scFV. Derived from hybridoma L121/133 scFv.DepositorInsertnNOS/NOS1 (Homo sapiens) recombinant scFV (NOS1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)
Plasmid#191647PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-dADAM17 (#1)
Plasmid#177259PurposeA knockout vector for the dog Adam17DepositorInsertA gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cys-60nmSAH-SPYcatcher
Plasmid#214840PurposeThis plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-60nmSAH-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKH118_keima-YIPF4
Plasmid#214971PurposeGolgiphagy flux reporter keima-YIPF4DepositorInsertkeima-YIPF4 (YIPF4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mhYFP
Plasmid#186532PurposeMonomeric hyperfolder YFP fluorescent protein: bacterial expression plasmidDepositorInsertMonomeric hyperfolder YFP
UseTags6xHisExpressionBacterialMutationhfYFP-S147P/L195M/V206KPromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-cilia-APEX
Plasmid#73186PurposeExpresses cilia-APEX in mammalian cellsDepositorInsertcilia-APEX (Nphp3 Mouse, Glycine max)
UseTagsinternal GFP tagExpressionMammalianMutationPromoterEF1alphaAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
MLV Gag-YFP
Plasmid#1813DepositorInsertGag (gag MuLV)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceMay 19, 2005AvailabilityAcademic Institutions and Nonprofits only