In Situ Capture of Chromatin Interactions by Biotinylated dCas9.
Liu X, Zhang Y, Chen Y, Li M, Zhou F, Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J
Cell. 2017 Aug 24;170(5):1028-1043.e19. doi: 10.1016/j.cell.2017.08.003. PubMed Article
Liu X, Zhang Y, Chen Y, Li M, Zhou F, Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J
Cell. 2017 Aug 24;170(5):1028-1043.e19. doi: 10.1016/j.cell.2017.08.003. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100547 | pEF1a-FB-dCas9-puro | Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein |
| 100548 | pEF1a-BirA-V5-neo | Expresses C-terminal V5-tagged BirA biotin ligase |
| 100549 | pSLQ1651-sgRNA(F+E)-sgGal4 | sgGal4-expressing vector modified from Addgene plasmid #51024 |