Addgene

We narrowed to 2,249 results for: PRK

Showing: 1441 - 1460 of 2249 results

Results per page:

Sort results by:

pDEST47-SARA-GFP

Plasmid

#67410

Purpose

Mammalian expression of hSara with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pmCherry-PH(Akt2)

Plasmid

#86596

Purpose

Expresses mCherry-tagged PH(WT) domain of human Akt2

Depositor

Ivan Yudushkin, Thomas Leonard

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2 PH domain (AKT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Amino acids 1 - 108

Promoter

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPA016 (delta 86-130 Parkin)

Plasmid

#102357

Purpose

expresses Rat Parkin with residues 86-130 deleted

Depositor

Kalle Gehring

Article

Sauve et al EMBO J. 2015 Oct 14;34(20):2492-505. doi

Insert

Parkin (Prkn Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

deleted residues 86 to 130

Promoter

tac

Available since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Raptor Halo

Plasmid

#112754

Purpose

express Raptor-Halo

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Raptor-Halo (RPTOR Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCL-HA-MAPKK1-WT

Plasmid

#40808

Depositor

Natalie Ahn

Article

Mansour et al Science. 1994 Aug 12;265(5174):966-70.

Insert

MAP2K1 (MAP2K1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
MAC_C_LMTK2

Plasmid

#187752

Purpose

MAC-tagged gene expression of human LMTK2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

LMTK2 (LMTK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-FRB:Akt2

Plasmid

#72899

Purpose

Recruitable Akt2 (LocaTOR2)

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Serine/threonine protein kinase Bβ (AKT2 Human)

Use

Lentiviral

Tags

FRB(T2098L)

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 T7 Jip1

Plasmid

#51699

Purpose

JIP-1 may suppress signal transduction by the JNK pathway"

Depositor

Roger Davis

Article

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jnk interacting protein 1 (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
pRc/RSV Flag MKK3

Plasmid

#14671

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK3 (MAP2K3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pBS PKC theta

Plasmid

#8426

Depositor

Frederic Mushinski

Article

Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

Insert

Mouse Protein Kinase C theta (Prkcq Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 22, 2005

Availability

Academic Institutions and Nonprofits only
pMTH PKC zeta

Plasmid

#8414

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C zeta (Prkcz Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pcdna3 Flag Jip2

Plasmid

#59027

Purpose

act as molecular scaffolds that mediate the activation of the JNK signaling pathway in vivo.

Depositor

Roger Davis

Article

Yasuda et al Mol Cell Biol. 1999 Oct;19(10):7245-54.

Insert

mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2 )

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
pC1-FGB (FlincG2)

Plasmid

#49204

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Article

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Insert

FlincG2 (PRKG1 Bovine, GFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 Flag Jnk1b1

Plasmid

#13753

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1b1 (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK5kin

Plasmid

#65245

Purpose

Expression of HA tagged ERK5 kinase domain in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5kin (MAPK7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 410-817

Promoter

CMV

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only
Erk1

Plasmid

#29578

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Erk1 (MAPK3 Human)

Use

Tags

FLAG, HIS6, and TEV

Expression

Bacterial

Mutation

K289N mutation present. Starting Met removed from…

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
Halo-Tev-Akt3 WT-2X Flag

Plasmid

#87607

Purpose

expresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of human Akt3 protein

Depositor

Yimon Aye

Insert

Akt3 isoform 2 (AKT3 Human)

Use

Zebrafish (danio rerio) expression

Tags

2 tandem flag tags and His-Halo-Tev

Expression

Mammalian

Mutation

Akt3 isoform 2

Promoter

SP6

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM9-MAPKK2-WT

Plasmid

#40805

Depositor

Natalie Ahn

Article

Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

Insert

MAP2K2 (MAP2K2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Wild type

Promoter

MSV-LTR

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pFKBPmCherry-Akt2(K14A)

Plasmid

#86597

Purpose

Expresses recruitable mCherry-tagged human Akt2(K14A) membrane-binding mutant

Depositor

Ivan Yudushkin, Thomas Leonard

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

FKBB-mCherry-Akt2(K14A) (AKT2 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Akt2(K14A/R25E)

Plasmid

#86595

Purpose

Expresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutant

Depositor

Ivan Yudushkin, Thomas Leonard

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A/R25E) (AKT2 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge