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Addgene

Erk1
(Plasmid #29578)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29578 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMCSG7
  • Backbone size w/o insert (bp) 5286
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Erk1
  • Species
    H. sapiens (human)
  • Mutation
    K289N mutation present. Starting Met removed from Erk1.
  • Entrez Gene
    MAPK3 (a.k.a. ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK)
  • Tags / Fusion Proteins
    • HIS6 (N terminal on backbone)
    • TEV (N terminal on backbone)
    • FLAG (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene plasmid# 14747 was used as template for Erk1.
Ligation-independent-cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Erk1 was a gift from Dustin Maly (Addgene plasmid # 29578 ; http://n2t.net/addgene:29578 ; RRID:Addgene_29578)
  • For your References section:

    Sequence determinants of a specific inactive protein kinase conformation. Hari SB, Merritt EA, Maly DJ. Chem Biol. 2013 Jun 20;20(6):806-15. doi: 10.1016/j.chembiol.2013.05.005. 10.1016/j.chembiol.2013.05.005 PubMed 23790491