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pHR_PGK_antiCD19_synNotch_TetRVP64

Plasmid

#79126

Purpose

Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch TetRVP64 Receptor (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

antiCD19_synNotch_TetRVP64

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7A Q67L

Plasmid

#28049

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Use

Tags

2xHA and EGFP

Expression

Mammalian

Mutation

Q67L

Promoter

Available since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

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moxGFP

Plasmid

#68070

Purpose

C-terminal fusion to moxGFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Type

Empty backbone

Use

Tags

moxGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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T7-RelA

Plasmid

#21984

Depositor

Warner Greene

Article

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Insert

NFkB p65 subunit (RELA Human)

Use

Tags

T7 gp10

Expression

Mammalian

Mutation

For more details for this expression construct,…

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
Flag-SIRT1 H363Y

Plasmid

#1792

Depositor

Michael Greenberg

Article

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

SIRT1 (SIRT1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

H363Y, deacetylase domain mutation

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only

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pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

Plasmid

#61593

Purpose

A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Inserts

hSaCas9
Chimeric guide for SaCas9

Use

AAV and CRISPR

Tags

HA, NLS, and OLLAS tag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET-28b-RfxCas13d-His

Plasmid

#141322

Purpose

Plasmid for bacterial expression and purification of RfxCas13d protein

Depositor

Miguel Angel Moreno-Mateos, Ariel Bazzini

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pZR071_SFFV-dCas9-mCherry-KRAB

Plasmid

#180264

Purpose

Lentiviral expression vector for dCas9-KRAB in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

KRAB and mCherry

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP GR

Plasmid

#47504

Purpose

Expresses glucocorticoid receptor in mammalian cells

Depositor

Alice Wong

Insert

Glucocorticoid receptor (NR3C1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pLEM415-ldhL-mRFP1

Plasmid

#99842

Purpose

Expression of RFP in Lactobacillus

Depositor

Sujin Bao

Article

Bao et al PLoS One. 2013;8(3):e60007. doi: 10.1371

Insert

monomeric red fluorescent protein

Use

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

ldhl

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-TRIM28

Plasmid

#45568

Depositor

Fanxiu Zhu

Article

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Insert

TRIM28 (TRIM28 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

G34S mutation in EGFP

Promoter

CMV

Available since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-CFP

Plasmid

#13030

Purpose

Mammalian expression vector for cloning your gene fused to CFP

Depositor

Doug Golenbock

Has Service

Cloning Grade DNA

Insert

cyan fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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myc-Rictor corrected

Plasmid

#11367

Purpose

Mammalian expression of myc tagged human RICTOR

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/opto-a1AR-EYFP

Plasmid

#20947

Purpose

Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Article

Airan et al Nature. 2009 Mar 18. ():.

Insert

opto-a1AR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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HDAC1 Flag

Plasmid

#13820

Purpose

Mammalian expression of human histone deacetylase 1 with flag tag

Depositor

Eric Verdin

Article

Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

Insert

HDAC1 (HDAC1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLII12Pglu-700uDelta6

Plasmid

#17866

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLII12Pglu-700uDelta6

Use

Tags

Citrine

Expression

Mammalian

Mutation

700uDelta6, ECFP insert in mglB

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 NSP3

Plasmid

#141257

Purpose

Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Has Service

Cloning Grade DNA

Insert

NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-U6-gRNA-CBh-mCherry

Plasmid

#91947

Purpose

Expression of empty gRNA cassette and mCherry

Depositor

Jimok Kim

Article

Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

Insert

mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLX-TRE-dCas9-KRAB-MeCP2-BSD

Plasmid

#140690

Purpose

Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

Cas9m4-KRAB-MeCP2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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HLTV-hTRIM21

Plasmid

#104973

Purpose

Purification of His-Lipoyl-hTRIM21 protein from bacteria

Depositor

Leo James

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Tripartite Motif containing 21 (TRIM21 Human)

Use

Tags

His-Lipoyl

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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