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pCMV6M-Pak1

Plasmid

#12209

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pLKO.1 3xHA-KillerRed-OMP25

Plasmid

#174544

Purpose

localizes KillerRed to mitochondria

Depositor

Minna Roh-Johnson

Article

Kidwell et al Elife. 2023 Mar 6;12:e85494. doi: 10.755

Insert

3xHA-KillerRed-OMP25

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLOX-TERT-iresTK

Plasmid

#12245

Purpose

2nd generation lentiviral vector for reversible immortalization of human primary cells

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

TERT, HSV1-TK (TERT Human)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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mApple-Alpha-5-Integrin-12

Plasmid

#54864

Purpose

Localization: Focal Adhesions, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

Alpha-5 Integrin (ITGA5 Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGL4.27_ARE/NRF2-SPE

Plasmid

#177775

Purpose

Luciferase reporter for ARE/NRF2

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

NRF2 (NFE2L2 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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Str-Ii_neomycin

Plasmid

#65312

Purpose

Cytoplasmic ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Use

Tags

Expression

Mammalian

Mutation

Streptavidin in its cytoplasmid part

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pLX304 YAP1

Plasmid

#193689

Purpose

Constitutive lentiviral expression of YAP1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHis APEX2-eIF4A1

Plasmid

#129645

Purpose

Express APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteria

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

eukaryotic translation initiation factor 4A1 (EIF4A1 Human)

Use

Tags

6xHis and APEX2

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Dcp1a

Plasmid

#153972

Purpose

Fluorescent protein fused to mRNA decapping enzyme 1a

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA decapping enzyme 1a (DCP1A Human)

Use

Tags

pAcGFP-C1

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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Emerin pEGFP-N2 (588)

Plasmid

#61985

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

Emerin (EMD Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR3__TACC3

Plasmid

#116818

Purpose

Lentiviral expression of FGFR3__TACC3 fusion

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR3__TACC3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI-CMV-GFP-TREX1 BLAST

Plasmid

#164228

Purpose

lentiviral plasmid for GFP-hTREX1

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-hTERT

Plasmid

#1773

Purpose

Retroviral expression of hTERT. Used to create immortalized cell lines.

Depositor

Bob Weinberg

Article

Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

Insert

hTERT (TERT Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASH

Plasmid

#125554

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Expression

Mutation

Truncated form: only the KASH domain of nesprin-…

Promoter

tetracycline responsive element

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pXAT2

Plasmid

#80494

Purpose

AAVS1 sgRNA expression vector

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 sgRNA-T2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL4B-pCMV7.1

Plasmid

#155023

Purpose

N-terminal 3xFLAG-tagged CUL4B in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 4B (CUL4B Human)

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

pCMV7.1

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta wt pcDNA3

Plasmid

#14753

Purpose

Mammalian expression of human GSK3 beta with HA tag

Depositor

Jim Woodgett

Article

He et al Nature. 1995 Apr 13. 374(6523):617-22.

Insert

GSK3 beta (GSK3B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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EGFP-Tubulin.K40Q

Plasmid

#32912

Depositor

Tso-Pang Yao

Article

Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

Insert

Tubulin (TUBA1B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K40Q

Promoter

CMV

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pEGFPC1-hVAP-A

Plasmid

#104447

Purpose

Expression of human VAP-A fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-rab11 DN

Plasmid

#12678

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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