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Plasmid#196873PurposeExpression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to Halo-Tag. Used to displace endogenous Akap9 from GolgiDepositorInsertHalo-Tag-Akap9 (128-425) (Akap9 Rat)
UseTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Myo 9a-GFP
Plasmid#134959PurposeExpression of rat Myo 9a (Myr7) in mammalian cells.DepositorInsertMyo 9a (rat) with 5' UTR (Myo9a Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB-GLUT4-7myc-GFP
Plasmid#52872Purposedetection and measurement of GLUT4 translocationDepositorInsertGLUT4 (Slc2a4 Rat, with human c-terminus)
UseRetroviralTags7x-myc (internal) and GFPExpressionMammalianMutationPromoterAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-KIF1A
Plasmid#102850PurposeOverexpresses KIF1A and cell fill TdTomatoDepositorInsertKIF1A (Kif1a Rat)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
PSD-95 FLAG
Plasmid#15463DepositorInsertPSD-95 (Dlg4 Rat)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
Syn-ATP
Plasmid#51819PurposeOptical reporter of presynaptic ATPDepositorInsertChimeric Synaptophysin-mCherry-Luciferase
UseTagsExpressionMammalianMutationWithin WT luciferase gene, changed Threonine 214 …PromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-lyso-pHoenix
Plasmid#70112PurposeExpression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomesDepositorInsertlyso-pHoenix (Cd63 Mouse, Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)
UseTagsArch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRX-No-HaloTag7-mGFP-LC3-mRFP
Plasmid#184902PurposeStable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic fluxDepositorInsertLC3B
UseTagsHaloTag7, monomeric EGFP, and monomeric RFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV mRuby3-Synapsin1a
Plasmid#187896PurposeFor labeling pre-synaptic terminalsDepositorInsertSynapsin1a
UseTagsmRuby3ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-bFosVC155
Plasmid#22013DepositorInsertbFosVC155
UseTagsHAExpressionMammalianMutationN/APromoterAvailable sinceOct. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt4
Plasmid#184505PurposeMammalian expression of Synaptotagmin 4 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 4 (Syt4 Rat)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dronpa-Homer1-C-18
Plasmid#57279PurposeLocalization: Membrane Adaptor, Excitation: 503, Emission: 518DepositorInsertHomer1 (Homer1 Rat)
UseTagsDronpaExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmCherry and superfolder GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s(alpha3beta5/G226A/A366S)
Plasmid#55797PurposeThis highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.DepositorInsertalpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationN271K, K274D, R280K, T284D, I285T, G226A, A366S i…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-mDlx-jGCaMP8s-WPRE
Plasmid#176755PurposejGCaMP8s expression in GABA-ergic interneurons under the control of mDlx enhancerDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromotermDlxAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKII-TRPV1-P2A-mCherry
Plasmid#200829PurposeTransduce excitatory neurons with AAV to express TRPV1 along with an mCherry fluorescence reporterDepositorInsertTRPV1-P2A-mCherry (Trpv1 Rat)
UseAAVTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Camk2a KI
Plasmid#131484PurposeEndogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGFP Cas
Plasmid#50729Purposemammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertCas (Bcar1 Rat)
UseTagsFLAG and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCit-4aa-SNAP25B
Plasmid#53235PurposeFluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.DepositorInsertSNAP-25B (Snap25 Rat)
UseTags4aa-linker and mCitrineExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Bsn KI
Plasmid#139664PurposeEndogenous tagging of Bassoon: N-terminal (amino acid position: L7)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only