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Addgene

pcDNA type III TGF beta receptor (R3-OFF)
(Plasmid #10913)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10913 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNAI neo
  • Backbone size w/o insert (bp) 7100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    R3-OFF
  • Alt name
    Type III TGF beta receptor
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    5000
  • Entrez Gene
    Tgfbr3 (a.k.a. betaglycan)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA type III TGF beta receptor (R3-OFF) was a gift from Bob Weinberg (Addgene plasmid # 10913 ; http://n2t.net/addgene:10913 ; RRID:Addgene_10913)
  • For your References section:

    Expression cloning and characterization of the TGF-beta type III receptor. Wang XF, Lin HY, Ng-Eaton E, Downward J, Lodish HF, Weinberg RA. Cell. 1991 Nov 15. 67(4):797-805. 10.1016/0092-8674(91)90074-9 PubMed 1657407