pcDNA type III TGF beta receptor (R3-OFF)
(Plasmid
#10913)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 10913 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNAI neo
- Backbone size w/o insert (bp) 7100
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameR3-OFF
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Alt nameType III TGF beta receptor
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SpeciesR. norvegicus (rat)
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Insert Size (bp)5000
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Entrez GeneTgfbr3 (a.k.a. betaglycan)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA type III TGF beta receptor (R3-OFF) was a gift from Bob Weinberg (Addgene plasmid # 10913 ; http://n2t.net/addgene:10913 ; RRID:Addgene_10913) -
For your References section:
Expression cloning and characterization of the TGF-beta type III receptor. Wang XF, Lin HY, Ng-Eaton E, Downward J, Lodish HF, Weinberg RA. Cell. 1991 Nov 15. 67(4):797-805. 10.1016/0092-8674(91)90074-9 PubMed 1657407
Map uploaded by the depositor.