pcDNA3-ALK7 T194D
(Plasmid
#80886)
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Purposemammalian expression of ALK7 T194D
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80886 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameALK7
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Alt nameACVR1C
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Alt nameactivin A receptor type 1C
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SpeciesR. norvegicus (rat)
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MutationT194D (constitutively active)
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Entrez GeneAcvr1c (a.k.a. Alk-7, Alk7, habrec1)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-ALK7 T194D was a gift from Aristidis Moustakas (Addgene plasmid # 80886 ; http://n2t.net/addgene:80886 ; RRID:Addgene_80886) -
For your References section:
Functional consequences of tumorigenic missense mutations in the amino-terminal domain of Smad4. Moren A, Itoh S, Moustakas A, Dijke P, Heldin CH. Oncogene. 2000 Sep 7;19(38):4396-404. 10.1038/sj.onc.1203798 PubMed 10980615