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Plasmid#68437PurposeTransient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.DepositorInsertTOP1 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U1Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI
Plasmid#115213PurposeExpresses RanCas13b from a SUMO-tagged bacterial expression constructDepositorInsertRanCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)
Plasmid#163074PurposeEntry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)DepositorInsertGrx1-roGFP1-iEER
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC155-pCR8-sgExpression
Plasmid#49045PurposeU6-driven sgRNA expressing cassette on a gateway donor vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV/3' Box_(GLuc)_TOP2
Plasmid#68435PurposeTransient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.DepositorInsertTOP2 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-sfGFP-MiniWhite
Plasmid#165910PurposeBlasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hNEUROD1 gRNA_1-MS2-Puro
Plasmid#192676PurposeLentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcriptionDepositorInsertHuman NEUROD1 activating gRNA #1 (NEUROD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC0067 PguCas13 (B11) His6-TwinStrep-SUMO-BsaI
Plasmid#115218PurposeExpresses PguCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPguCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[Spinach2]
Plasmid#68430PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing the "Spinach2" aptamer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-TV
Plasmid#158409PurposeCRISPR-dpcoCas9 fused with TV activator for transcriptional activationDepositorInsertdpcoCas9-TV(6xTAL-2xVP64)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_TOP1
Plasmid#68423PurposeTransient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoterDepositorInsertTOP1 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
AKARdual
Plasmid#99869PurposeMeasure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrateDepositorInsertAKAR4
UseTagsLSSmOrange and mKate2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV/MASC_(GLuc)_INT
Plasmid#68440PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_hH1
Plasmid#102847PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_hH1
UsePcr template for stagr insertsTagsExpressionMutationPromoterU6Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-mNeurog2 gRNA_1-MS2-Puro
Plasmid#192680PurposeLentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcriptionDepositorInsertMouse Neurog2 activating gRNA #1 (Neurog2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_hU6
Plasmid#102843PurposeCan be used as PCR template for a STAgR reactionDepositorInsertgRNAScaffold_hU6promoter
UseAs pcr templateTagsExpressionMutationPromoterAvailable sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:Lucia:bgH
Plasmid#135293PurposeTranscriptional unit for constitutive expression of Lucia luciferase in mammalian cellsDepositorInsertCMV:Lucia:bGH
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK-puromycin-AflII
Plasmid#106404PurposeEmpty guide RNA cloning vector with an AflII site added from Addgene 41824DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6Available sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro
Plasmid#192681PurposeLentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcriptionDepositorInsertMouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgSetd2#1/Cre
Plasmid#89649PurposeExpresses a Setd2-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Setd2 (Setd2 Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only