Purpose
Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
Inserts
gRNA scaffold
zCas9
Use
CRISPR; Plant binary vector
Tags
3x FLAG and NLS
Expression
Plant
Mutation
Zea mays codon-optimized Cas9
Promoter
EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
Available since
Jan. 14, 2016
Availability
Academic Institutions and Nonprofits only