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Plasmid#186835PurposeExpresses FLAG-BMAL1-S513A/S515A/S516A in mammalian cellsDepositorInsertbmal1 (Bmal1 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine 513/515/516 to AlaninePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-8
Plasmid#172772PurposeBacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-8
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Opto-M1R
Plasmid#106069PurposeExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-M1R; Opto-M1R; F11; 722)DepositorInsertOpto-M1R (CHRM1 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHCA/GAL4(1-93).ER.VP16
Plasmid#108216PurposeYeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domainDepositorInsertGal4 DBD-ERalpha HBD-VP16
UseTagsExpressionYeastMutationERalpha HBD has G400V mutationPromoterADH1Available sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
UseTagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7Available sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
VRK1-dTag-puro
Plasmid#199651PurposeExpresses VRK1 C-term. Tagged with dTAGDepositorInsertVRK1 (VRK1 Human)
UseLentiviralTagsdTAGExpressionMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ3 SHP2 D61A
Plasmid#8320DepositorInsertSHP2 (PTPN11 Human)
UseTagsExpressionMammalianMutationD61A (activated mutant)PromoterAvailable sinceJune 17, 2005AvailabilityAcademic Institutions and Nonprofits only -
pUBC_hsPUM1-iRFP670
Plasmid#107609PurposeTransient expression of Pumilio1, fused to iRFP670 in mammalian cells, under a hUBC promoterDepositorInserthsPUM1-iRFP670
UseSynthetic BiologyTagsiRFP670ExpressionMammalianMutationPromoterhuman UBCAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-K14R
Plasmid#185759PurposeBacterial expression of His6-Ubc9-K14RDepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationK14RPromoterT7Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-3XHA-EGFP-OMP25
Plasmid#83356PurposeFor tagging mitochondria with HA epitopes (HA-MITO construct)DepositorInsert3XHA-EGFP-OMP25
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFD7
Plasmid#73916PurposegRNA vector for AsCpf1DepositorInserttRNA:hAsCpf1-gRNA:tRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Opto-D2R
Plasmid#106068PurposeExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-D2R; Opto-D2R; F10; 721)DepositorInsertOpto-D2R
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-D1R
Plasmid#106067PurposeExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-D1R; Opto-D1R; F9; 720)DepositorInsertOpto-D1R
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
ASC-CASP1 Octamer
Plasmid#164032PurposeBacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamerDepositorUseTagsHis6-MBP-TEVExpressionBacterialMutationW169G (ASC), G20K (CASP1)PromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-mGFP-pA(AV222)
Plasmid#117858PurposeThis plasmid expresses membrane anchored GFP to facility axon morphology study.DepositorInsertmGFP
UseAdenoviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
SPOCK2_22-424_AviN_HisC
Plasmid#195877PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertSPOCK2 (SPOCK2 Human)
UsePfhmsp-avin-lic-c, baculovirus expressionTagsExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHLM
Plasmid#64168Purposeharbours marker genes HIS3, LEU2 and MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-Epac1 SAAX
Plasmid#118313PurposeExpression of human Epac1DepositorInsertEpac1 SAAX (RAPGEF3 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-G-Hras
Plasmid#180580PurposeddGFP sensor of HrasDepositorInsertddGFP sensor of Hras
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag TTK
Plasmid#20659DepositorInsertTTK (TTK Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS*-PrpoN-Cro-PBBa_J23106-geneIII
Plasmid#134353PurposePhagemidDepositorInsertCro
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-1-333
Plasmid#104194PurposeExpresses human AR aa 1-333 in pGEX-5x-1 bacteral expression vector with GSTDepositorInserthuman androgen receptor aa 1-333 (AR Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pKK-TEV-MBP
Plasmid#105787PurposeExpression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-MBPExpressionMammalianMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-174-566
Plasmid#104197PurposeExpresses human AR-174-566 in pGEX-3x bacterial expression vector as fusion protein with GSTDepositorInserthuman androgen receptor aa 174-566 (AR Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEW100cre_del_tetO
Plasmid#24019DepositorTypeEmpty backboneUseTrypanosome transient expressionTagsCre RecombinaseExpressionMutationPromoterAvailable sinceMay 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
myr-FLAG-PIK3CA-pcw107
Plasmid#64605Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertPIK3CA (PIK3CA Bovine)
UseLentiviralTagsFLAG and myristoylatedExpressionMutationPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
acta1-mRuby2-dGBP
Plasmid#163855PurposeZebrafish muscle specific expression of mRuby2-dVHH[GFP]DepositorInsertdVHH[GFP]
UseIn vitro transcription of rnaTagsRuby2ExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM10 with rat FL Annexin A11
Plasmid#164496PurposeFor bacterial expression of rat His-tagged FL Annexin A11DepositorInsertrat FL Annexin A11 (Anxa11 Rat)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pblic-neo TRX1
Plasmid#110919PurposeTo overexpress Thioredoxin-1DepositorInsertTRX1 (TXN Human)
UseRetroviralTagsExpressionMutationPromoterunknownAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only