Skip to main content
Addgene
Showing: 1241 - 1260 of 14475 results

  1. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

    Plasmid
    #228530
    Purpose
    Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.
    Depositor
    Roman Jerala
    Article
    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80
    Insert
    myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Synthetic, Human, Photinus pyralis)
    Use
    Tags
    HA and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    228530-plasmid-map-sequence-id-456690

    Enlarge

  5. pHis-TEV-Nsp15

    Plasmid
    #187656
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004
    Insert
    Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7lac
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    187656-plasmid-map-sequence-id-388768

    Enlarge

  6. TALEN Backbone

    Plasmid
    #31179
    Depositor
    Feng Zhang
    Insert
    TALE Nuclease Backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  7. pBAD33.1-DpnII

    Plasmid
    #127413
    Purpose
    Expresses DpnII restriction endonuclease in bacteria
    Depositor
    Douglas Feldman
    Insert
    DpnII
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    127413-plasmid-map-sequence-id-247669

    Enlarge

  8. pOpen-T7EI

    Plasmid
    #165540
    Purpose
    DNA Endonuclease; catalyzes cleavage of DNA mismatches and non-beta DNA structures (Holliday junctions and cruciform).
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    T7EI (T7 Endonuclease I)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165540-plasmid-map-sequence-id-339262

    Enlarge

  9. pmCer-TopBP1

    Plasmid
    #211412
    Purpose
    mammalian expression of human TopBP1 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    TopBP1 (TOPBP1 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211412-plasmid-map-sequence-id-420515

    Enlarge

  10. pmCer-RecQL4

    Plasmid
    #211400
    Purpose
    mammalian expression of human RecQL4 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    RecQL4 (RECQL4 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211400-plasmid-map-sequence-id-420508

    Enlarge

  11. pNIC28-Bsa4-iLOVf

    Plasmid
    #63722
    Purpose
    LIC E coli expression plasmid to produce TEV cleavable His tagged iLOV fusion proteins
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    TEE, iLOV, 6xHis, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63722-plasmid-map-sequence-id-106345

    Enlarge

  12. 371-Bc

    Plasmid
    #97028
    Purpose
    yORF-tev-His6
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97028-plasmid-map-sequence-id-182499

    Enlarge

  13. 371-B

    Plasmid
    #97027
    Purpose
    His6-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97027-plasmid-map-sequence-id-182504

    Enlarge

  14. 371-R

    Plasmid
    #97033
    Purpose
    StrepII-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    StrepII tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97033-plasmid-map-sequence-id-182501

    Enlarge

  15. 371-L

    Plasmid
    #97031
    Purpose
    FLAG-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    FLAG tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97031-plasmid-map-sequence-id-182492

    Enlarge

  16. RAB5AA-c003

    Plasmid
    #175475
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-N215.
    Depositor
    Opher Gileadi
    Insert
    RAB5A (RAB5A Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175475-plasmid-map-sequence-id-353172

    Enlarge

  17. RABEP1A-c019

    Plasmid
    #175477
    Purpose
    Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    RABEP1 (RABEP1 Human)
    Use
    Tags
    TEV-TwinStrep
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175477-plasmid-map-sequence-id-353250

    Enlarge

  18. RDXB-c004

    Plasmid
    #175479
    Purpose
    Protein expression in bacterial cells. FERM domain, M1-A321.
    Depositor
    Opher Gileadi
    Insert
    RDX (RDX Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175479-plasmid-map-sequence-id-353214

    Enlarge

  19. MSNA-c000

    Plasmid
    #175465
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-M577
    Depositor
    Opher Gileadi
    Insert
    MSN (MSN Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175465-plasmid-map-sequence-id-353148

    Enlarge

  20. TBXTA-c005

    Plasmid
    #139754
    Purpose
    For protein expression/crystallography (PDB:6F58)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-S224
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139754-plasmid-map-sequence-id-296505

    Enlarge

Showing: 1241 - 1260 of 14475 results