pS97splitRBP_S1dC2
(Plasmid
#183769)
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PurposeExpresses SHIP1-delta-C2-Enzyme domain flanked by RBP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepKan S97Split
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Backbone manufacturerN/A
- Backbone size w/o insert (bp) 6067
- Total vector size (bp) 7054
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSHIP1deltaC2
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Alt nameSHIP1dC2
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Alt nameINPP5D deltaC2
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SpeciesH. sapiens (human)
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Insert Size (bp)1022
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MutationSHIP1deltaC2 includes aa N393 to aa Q730 only
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GenBank IDNM_001017915
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Entrez GeneINPP5D (a.k.a. SHIP, SHIP-1, SHIP1, SIP-145, hp51CN, p150Ship)
- Promoter T7lac
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Tags
/ Fusion Proteins
- 6HIS (N terminal on backbone)
- RBP (N-terminal) w/HRV3C cleavage site (N terminal on backbone)
- RBP (C-terminal) w/HRV3C cleavage site (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byVector backbone obtained from Stewart Loh, SUNY-Upstate Medical University
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pS97splitRBP_S1dC2 was a gift from William Kerr (Addgene plasmid # 183769 ; http://n2t.net/addgene:183769 ; RRID:Addgene_183769) -
For your References section:
LRBA Deficiency Can Lead to Lethal Colitis That Is Diminished by SHIP1 Agonism. Sudan R, Fernandes S, Srivastava N, Pedicone C, Meyer ST, Chisholm JD, Engelman RW, Kerr WG. Front Immunol. 2022 May 4;13:830961. doi: 10.3389/fimmu.2022.830961. eCollection 2022. 10.3389/fimmu.2022.830961 PubMed 35603158