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Showing: 1221 - 1240 of 1302 results

  5. pcU6_3 MS2 sgRNA

    Plasmid
    #92394
    Purpose
    Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    chick U6.3
    Availability
    Academic Institutions and Nonprofits only
    92394-plasmid-map-sequence-id-191866

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  7. pAP14

    Plasmid
    #186262
    Purpose
    wgaA (a.k.a expA1) and wgaB (a.k.a expA23), bicistronic, under light light responsive PEL222 promoter and EL222 under constitutively active LacIq promoter
    Depositor
    Yongku Cho
    Article
    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35
    Inserts
    wgaAB bicistronic
    EL222 (ELI_04755 Erythrobacter litoralis)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    LacIq (CGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCC…
    Availability
    Academic Institutions and Nonprofits only
    186262-plasmid-map-sequence-id-369317

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  8. pAWPSG-Ptet-nCas9-BE

    Plasmid
    #195739
    Purpose
    Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptet(Tetracycline inducible protein)
    Availability
    Academic Institutions and Nonprofits only
    195739-plasmid-map-sequence-id-386424

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  15. pSW002-Pc-TorA(sp)-mNeonGreen

    Plasmid
    #205020
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorA-mNeonGreen
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Availability
    Academic Institutions and Nonprofits only
    205020-plasmid-map-sequence-id-407993

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  16. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid
    #205021
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorT-mNeonGreen
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Availability
    Academic Institutions and Nonprofits only
    205021-plasmid-map-sequence-id-407999

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  19. pcDNA4/TO-bio-myc-cCE

    Plasmid
    #112711
    Purpose
    For tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cells
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    Rngtt (Rngtt Mouse)
    Use
    Tags
    HIV Rev nuclear localization signal (NES), Stop c…
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV with Tet repressor binding sites
    Availability
    Academic Institutions and Nonprofits only
    112711-plasmid-map-sequence-id-217051

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  20. pcDNA3.1/TO-myc-BirA*-cCE

    Plasmid
    #112713
    Purpose
    For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    Rngtt (Rngtt Mouse)
    Use
    Tags
    BirA* (BirA R118G), HIV Rev nuclear localization …
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    112713-plasmid-map-sequence-id-217067

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Showing: 1221 - 1240 of 1302 results