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We narrowed to 37 results for: tetracycline

Showing: 1 - 20 of 37 results

  1. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...reverse tetracycline-controlled transactivator) is normally inactive and cannot bind the tetracycline response... inducible promoters. The positive inducible tetracycline ON (Tet-On) system, a versatile tool developed...response elements (TRE) in a promoter. Tetracycline and its derivatives serve as inducing agents to allow...Addgene.org: Inducible Promoters for Bacteria Tetracycline (Tet) Inducible Systems at Addgene Cre-lox Guide...

  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...its native context, the tetracycline repressor (TetR) can bind to the tetracycline operator sequences (TetO...preventing transcription. In the presence of tetracycline (Tet), TetR preferentially binds Tet over the...repressible system, Gossen and Bujard created the tetracycline-controlled transactivator (tTA). They fused ...TRE)), allowing transcription to proceed. When tetracycline or one of its derivatives is added, it binds...information on Addgene Tet plasmids, see our Tetracycline resource page. Cumate Switch Massie et. al developed...control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S...Signals FLEx Vectors Resources at Addgene.org Tetracycline Collection ...

  3. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...by titration with different amounts of tetracycline. Tetracycline regulation is reversible (when the drug...with more elements of control. Tet promoters Tetracycline-dependent promoters are engineered by placing...constitutively silenced proteins. Addition of tetracycline or one of its analogs will induce these transcriptional...transcriptional level.     Fig. 2 Tetracycline on/off promoters and modes of action Check out...

  4. Tetracycline Inducible Expression

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    Collection
    ... Plasmid Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression...Stem Cell Research Plasmids Collection Tetracycline Off Tetracycline On Experimental Considerations Tet Plasmids...shown. Tetracycline Off (Tet-Off) The first major advance was the Tet-Off system. A tetracycline-controlled...presence of tetracycline, tTA unbinds from the TRE, resulting in reduced gene expression. Tetracycline Off is...binds the TRE in the presence of tetracycline. A new reverse tetracycline-controlled transactivator (rtTA... absence of tetracycline, rtTA is unable to bind the TRE. In the presence of tetracycline, rtTA binds ...applications. Use of Tetracycline or a Derivative Doxycycline, a derivative of tetracycline, is a preferred...

  5. Plasmids 101: Antibiotic Resistance Genes

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    Blog Post
    ...permeability Bactericidal 10-100 ug/mL Tetracycline tetracyclin Binds 30S ribosomal subunit; inhibits...in foil if a light-sensitive antibiotic like Tetracycline is used. Keep in mind that some E. coli strains...

  6. AAV Molecular Tools

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    Collection
    ...Tools Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Tetracycline Transactivators... Tools These AAV encode tetracycline-inducible tools/controls and tetracycline transactivators that can...can be used with tetracycline (tet)-inducible expression systems . ID Name Expression System Activity ...Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression...

  7. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...not recommended for toxic proteins BL21-A1 Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG...T7RNAP-tetA General protein expression  BLR (DE3) Tetracycline RecA-deficient; best for plasmids with repetative...proteins Origami2 (DE3)** Streptomycin and Tetracycline Contains highly active thioredoxin reductase...

  8. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ... of XFPs in the original method, we used the Tetracycline trans-activator system to boost the expression...expression levels, and we named the system Tetbow (Tetracycline trans-activator Brainbow) (Sakaguchi et al.,...

  9. Plasmids 101: Common Lab E. coli Strains

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    Blog Post
    ... streptomycin Tn10   Confers resistance to tetracycline Additionally, Table 2 provides a quick reference...galE15 galK16 rpsL(StrR) endA1 λ- XL1 Blue Tetracycline Blue/white screening and routine cloning, nalidixic...lacIq Δ(lacZ)M15] hsdR17(rK- mK+)  XL10 Gold Tetracycline and Chloramphenicol High competency cloning ...

  11. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...splice acceptor. TRE General expression mRNA Tetracycline response element promoter Inducible with Tetracyline...promoter with low basal activity and several tetracycline operators. Transcription can be turned on or...

  12. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...used inducible lentiviral vector system is the tetracycline (Tet)-regulated system. You can choose either...promoter in your transfer vector. In the absence of tetracycline or a derivative such as Doxycycline (Dox), the...

  13. AAV Viral Preps

    Type
    Collection
    ...Spagehetti Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell...

  17. Retrovirus Plasmids

    Type
    Collection
    ...resistance. Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in...
Showing: 1 - 20 of 37 results