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Plasmid#48662PurposeBacterial ST1 repression YFP reporter: protospacer BDepositorInsertST1 prototspacer B/PAM with reporter EYFP
UseCRISPRTagsExpressionMutationPromoterlambda pR promoterAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLigify_LSorbose
Plasmid#215562PurposeExpresses GFP in response to L-SorboseDepositorInsertsGFP mut2
sorR
UseTagsExpressionBacterialMutationPromoterP250 and PsorrAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLigify_Isoeugenol
Plasmid#215563PurposeExpresses GFP in response to IsoeugenolDepositorInsertsGFP mut2
iemR
UseTagsExpressionBacterialMutationPromoterP250 and PiemrAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLigify_4Vinylphenol
Plasmid#215564PurposeExpresses GFP in response to 4-VinylphenolDepositorInsertsGFP mut2
vprR
UseTagsExpressionBacterialMutationPromoterPvprrAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor_RB-TnV2_pJEx
Plasmid#213908PurposeBarcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sitesDepositorInsertmariner transposon with kan resistance and EilR/pJEx and FseI-SbfI sites for barcode insertion
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
WNV NS2B-NS3 protease (based on PDB 2IJO, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein
Plasmid#204799PurposeBacterial expression of a fusion of West Nile NS2B-NS3 proteinsDepositorInsertWest Nile NS2B-NS3 fusion
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein
Plasmid#204797PurposeBacterial expression of a fusion of West Nile NS2B-NS3 proteinsDepositorInsertWest Nile NS2B-NS3 fusion
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pBD21_035
Plasmid#109152PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFPDepositorInsertsmRFP
EGFP
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH131
Plasmid#136550PurposecrRNA backbone for FnCas12a/CmsI nucleases, combine with OsU3 promoter module (pFH39)DepositorInsertcrRNA backbone for FnCas12a/CmsI nucleases, combine with OsU3 promoter module (pFH39)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH130
Plasmid#136549PurposecrRNA backbone for FnCas12a/CmsI nucleases, combine with OsU6-2 promoter module (pFH37)DepositorInsertcrRNA backbone for FnCas12a/CmsI nucleases, combine with OsU6-2 promoter module (pFH37)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
S5_EcoRV_Ef1a_AscI
Plasmid#128469PurposeEf1a promoterDepositorInsertEf1a promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S11_EcoRV_CAMKIIA_AscI
Plasmid#128475PurposeCAMKII1 promoterDepositorInsertCAMKII1 promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S10_EcoRV_hcSYN_AscI
Plasmid#128474PurposehcSYN promoterDepositorInserthcSYN promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK010
Plasmid#128227Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 4DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 4
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK011
Plasmid#128228Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 5DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 5
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK012
Plasmid#128229Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 6DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 6
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH87
Plasmid#128200PurposesgRNA backbone for StCas9, combine with TaU3 promoter module (pFH33)DepositorInsertsgRNA backbone for StCas9, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH89
Plasmid#128202PurposecrRNA backbone for LbCas12a, combine with TaU3 promoter module (pFH33)DepositorInsertcrRNA backbone for LbCas12a, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only