....   Kanamycin Kanamycin, or kan, is an aminoglycoside which inhibits protein synthesis. The kan resistance...resistance gene, NPTII, facilitates phosphorylation of kan, which inactivates it. The NPTII gene confers resistance...decorative" title="Check icon" %} Satellite colonies Kanamycin Excellent No Bacteria and eukaryotes (G418) {%...

...precise genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi, and Eric A. ... PMID: 28356322 PubMed Central PMCID: PMC5495063 Kan et al. use a GFP-BFP conversion system to assess ...

... as stabs at room temperature. ID Name 13421 pAC-Kan-alphaGal4 13422 pBAC-lacZ 13424 KJBAC1 strain 21869...

...previously described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee et al.(1...3xFlag, Protein A, or V5), and selection marker (KanMX, HphMX, or His3MX).  Each PCR product from this ...

...instead use G418. This can be confusing since the neo/kan gene confers G418 resistance; however, like gentamicin...

... Hygro Yang pKSE401 AtU6-26 yes, cut S. pyogenes Kan Chen pRGE31 rice snoRNA U3 BsaI none S. pyogenes ...

...serious-  competition with our corn toss boards, Kan Jam, and Ladder Ball (I finally discovered my hidden...

...Clover - Mammalian Expression pFA6a-link-yoClover-Kan - Yeast Expression dClover2 505 515 Dimer (A206K)...C1 - Mammalian Expression pFA6a-link-yoTagRFP657-Kan - Yeast Expression smURFP + BV 642 670 32 39 min ...Bacterial Expression tandem PA-GFP-KanR - Yeast Expression pFA6a-PA-GFP-kanMX6 - Yeast Expression pFA6a-link-yEPAGFP-CaUra3...

...PubMed PMID: 1195397. 2. Chang, Judy C., and Yuet Wai Kan. "A sensitive new prenatal test for sickle-cell anemia...

...Basta Chen pKSE401 62202 Plant yes, cut S. pyogenes Kan Chen pHUE411 62203 Plant yes, cut S. pyogenes Hyg...Jackson pMZ376 74213 Yeast rrk1 yes, cut S. pyogenes KanMX Zaratiegui pMZ377 74214 Yeast rrk1 yes, cut S. pyogenes...

...Cre rTH AAV Wilson 108454 mCherry-p2A-CreERT2-FRT-kan-FRT For in vitro transcription of Cre or to recombine...; Targeting vector for Sox1 locus CAG Mammalian Okano 153207 pAM-AAV-mSncg-Cre Retinal ganglion cell-specific...

...with the cells’ endogenous ubiquitin ligase, the Kanemaki lab was able to translate AID to yeast, as well...Nishimura et al., 2009, Natsume et al., 2016).  The Kanemaki lab previously developed a CRISPR/Cas9 based method...essential proteins (Natsume et al., 2016). Now, the Kanemaki lab has created a new toolkit of plasmids for ...create conditional human cell lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor...

...cleavage, KanR 26101 pET28GST-LIC EF456739 GST-tag and hexahistidine tag with Thrombin cleavage, KanR 26096...cleavage, KanR 26105 pNIC-CTHF EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, KanR 26106 pNH-...MHL EF456735 Hexahistidine tag with TEV cleavage, KanR 26098 pCW-LIC EF460848 No tag, double ptac promoter...Hexahistidine tag, thioredoxin with TEV cleavage, KanR 26107 pNIC-ZB GU452710 Hexahistidine tag , Z-basic...

...serotype can affect spread from the injection site  (Kanaan et al., 2017), axonal transport  (Murlidharan et...cells12050785 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-associated viral vectors...5), 1–41. https://doi.org/10.3390/cells12050785 Kanaan, N. M., Sellnow, R. C., Boye, S. L., Coberly, B...pharmaceutics13050750 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-associated viral vectors...

...AAV.cc81 Cap Asokan 199746 pAAV.cc84-mut AAV.cc84 Expresses AAV2 Rep and AAV.cc84 Cap Asokan 200257 pArk313...203532 iCAP-BI-28-KanR AAV-BI28 RepCap for AAV production Deverman 203533 iCAP-BI-48-KanR AAV-BI48 RepCap...203534 iCAP-BI-49-KanR AAV-BI49 RepCap for AAV production Deverman 203535 iCAP-BI-62-KanR AAV-BI62 RepCap...RepCap for AAV production Deverman 203536 iCAP-BI-65-KanR AAV-BI65 RepCap for AAV production Deverman 205991...

...signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes may prove challenging...and reliable imaging and assay results (Hayashi-Takanaka et al., 2014)​​. Conjugates with lower DOL tend...intracellular fluorescence live imaging ​​(Hayashi-Takanaka et al., 2014). Dyes can also vary in their brightness...994. https://doi.org/10.1038/nmeth.4403 Hayashi-Takanaka, Y., Stasevich, T. J., Kurumizaka, H., Nozaki,...

...backbone contains a kanamycin resistance cassette, you would plate the cells on kanamycin-containing agar..... Only cells carrying plasmids that contain a kanamycin resistance cassette will grow. However, often ...

...activity after ischemic brain injury Dr. Gohar Tsakanova, Institute of Molecular Biology of the NationalAcademy...Sciences of the Republic of Armenia, Armenia Gohar Tsakanova is a Senior Researcher who investigates the molecular...two-photon laser scanning microscope will allow Tsakanova to image cortical neurons and study changes in...

...Structure of a retrovirus. Image re-used from Dong & Kantor, 2021 under CC-BY license. Figure 2 gives a...1406488. PubMed Central PMCID: PMC372876 Dong, W., & Kantor, B. (2021). Lentiviral Vectors for Delivery of ...PubMed Central PMCID: PMC4515362 Robinson, W. S., Pitkanen, A., & Rubin, H. (1965). The nucleic acid of the...

... based on a bacterial haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand. The current...  (TMP-tag) Few Easy HaloTag 295 Chloralkane (CA) Many Easy CLIP-tag 182 Benzylcytosine...