WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein
(Plasmid
#204797)
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PurposeBacterial expression of a fusion of West Nile NS2B-NS3 proteins
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 204797 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepNIC-Kan-R
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameWest Nile NS2B-NS3 fusion
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SpeciesWest Nile
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 204797 ; http://n2t.net/addgene:204797 ; RRID:Addgene_204797)