pAK011
(Plasmid
#128228)
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Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 5
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128228 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57-Kan-mini
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Backbone manufacturerGenScript
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Vector typePlant Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAK011 was a gift from Vladimir Nekrasov (Addgene plasmid # 128228 ; http://n2t.net/addgene:128228 ; RRID:Addgene_128228) -
For your References section:
A modular cloning toolkit for genome editing in plants. Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. 10.1186/s12870-020-02388-2 PubMed 32326901