We narrowed to 15,731 results for: LEA

1. pCS2+-drAgo2

   Plasmid

   #221711

   Purpose

   Expression of zebrafish Ago2 in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drAgo2 (ago2 Zebrafish)
   

   Use

   Fish expression

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMCSG7-10xHis-ecRNHI*

   Plasmid

   #219523

   Purpose

   bacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene

   Depositor

   Arthur Palmer

   Article

   Bhattacharya et al J Biomol NMR. 2024 Jun 10. doi: 10.1007/

   Insert

   cysteine-free variant of the E. coli ribonuclease HI gene
   

   Tags

   N terminal 10xHis Tag

   Expression

   Bacterial

   Mutation

   Cysteine free variant

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. KBB430

   Plasmid

   #185091

   Purpose

   NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   First nuclear localization signal mutated (amino …

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. KBB428

   Plasmid

   #185090

   Purpose

   NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   First nuclear localization signal mutated (amino …

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAG

   Plasmid

   #130684

   Purpose

   Expresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-11E.12D-14.47M-48 (SMCHD1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D453_F489del, E653_G1960del

   Promoter

   CMV

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG

   Plasmid

   #130683

   Purpose

   Expresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D409_E452del, E653_G1960del

   Promoter

   CMV

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR8_TaNAM-A1

   Plasmid

   #124574

   Purpose

   TaNAM-A1 CDS in the Gateway-compatible pCR8 vector.

   Depositor

   Cristobal Uauy

   Article

   Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

   Insert

   TaNAM-A1
   

   Use

   Topo gateway cloning vector

   Promoter

   None

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Banshee-ShCit-A

   Plasmid

   #85216

   Purpose

   Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

   Depositor

   Alex Minella

   Article

   Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

   Insert

   shCit-A
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Banshee-ShCit-B

   Plasmid

   #85217

   Purpose

   Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

   Depositor

   Alex Minella

   Article

   Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

   Insert

   shCit-B
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. TALEN-CCR5L18-NtermN3

    Plasmid

    #51448

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with N3 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) N3 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Mutation

    K150Q, K153Q, and R154Q

    Promoter

    CMV

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. TALEN-CCR5R18-NtermN3

    Plasmid

    #51449

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with N3 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) N3 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K150Q, K153Q, and R154Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-eCas9

    Plasmid

    #140237

    Purpose

    Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    EF-1a core

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPgiCas13b-gRNA-1

    Plasmid

    #184834

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPgiCas13b-gRNA-NT

    Plasmid

    #184835

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. LBR EGFP

    Plasmid

    #128150

    Purpose

    Expression of lamin B receptor

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Ellenberg et al J Cell Biol. 1997 Sep 22. 138(6):1193-20

    Insert

    lamin B receptor (LBR Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3 V5 mDispatched WT 2x HA

    Plasmid

    #126410

    Purpose

    Expression of V5 Dispatched WT HA in mammalian cell lines.

    Depositor

    Stacey Ogden

    Article

    Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

    Insert

    m-Dispatched1 (Disp1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    V5 iserted between A106 and A107

    Available Since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 HisC EXO1b

    Plasmid

    #68268

    Purpose

    Mammalian expression of human EXO1

    Depositor

    Stefano Ferrari

    Article

    El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

    Insert

    EXO1 (EXO1 Human)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    b form, codons 1-846. Please see depositor commen…

    Promoter

    CMV

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pM9-BetaGal-8C

    Plasmid

    #172489

    Purpose

    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    M9-BetaGal-8C
    

    Tags

    6xHis and M9 nuclear localization sequence (from …

    Expression

    Bacterial

    Mutation

    BetaGal has two cysteine mutations (C77A, C1022S)…

    Promoter

    T7

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+-FLAG3-drAgo2

    Plasmid

    #221712

    Purpose

    Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2 (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-Golgin-84-TEV #709#243

    Plasmid

    #42108

    Depositor

    Ayano Satoh

    Article

    Koreishi et al Antibody Technology Journal. 2011;1:5-11

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    inserted ENLYFQG b/w 300 and 301

    Promoter

    CMV

    Available Since

    Feb. 20, 2013

    Availability

    Academic Institutions and Nonprofits only