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Plasmid#180273PurposeLentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassetteDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1-MS2-HA-HsStaufen2-T1_V
Plasmid#147741PurposeMammalian Expression of HsStaufen2-T1DepositorInsertHsStaufen2-T1 (STAU2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBA560
Plasmid#186236PurposepTet-eLbuCas13a crRNA entry vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE
Plasmid#60906PurposeThe plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imagingDepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3xnls-mScarlet3_N1
Plasmid#189775PurposeConstruct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3DepositorInsert3xnls-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
hRAP-pEZT-BM
Plasmid#177986PurposeExpression human RAP containing a C-terminal 6xHis tag in mammalian cellsDepositorInsertLDL receptor related protein associated protein 1 (LRPAP1 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab7A
Plasmid#28047DepositorInsertRab7A (RAB7A Human)
UseTags2xHA and EGFPExpressionMammalianMutationPromoterAvailable sinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENN496_miniCRISPRcharm_dSaCas9
Plasmid#220837Purposemini CRISPRcharm expression in mammalian cellsDepositorInsertmini CRISPRcharm
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST
Plasmid#208048PurposeEnables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidinDepositorInsertBirAopt
UseLentiviralTagsExpressionMutationBirAopt is human-codon-optimized BirA (PMID 15707…PromoterEFSAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)
Plasmid#209077PurposeNgn2 mediated cortical neuron induction, dox induction is includedDepositorInsertsUseTagsExpressionMammalianMutationPromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIVM_26
Plasmid#204501PurposeExpression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)DepositorUseTagsExpressionBacterialMutation0PromoterAmpRAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
secreted BirA-Flag
Plasmid#64395PurposeFlag-tagged biotinylation enzyme for co-expression with bio-proteinsDepositorInsertBirA (birA E. coli)
UseTagsFlag tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLHKI-pGFPN3
Plasmid#21917DepositorInsertFull length human HKI in pGFP-N3 (HK1 Human)
UseTagsGFPExpressionMammalianMutationThis is full length human HKI in pGFP-N3PromoterAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag
Plasmid#209652PurposeExpression of iATPSnFR2, medium affinityDepositorInsertiATPSnFR2.A95K.HaloTag
UseAAVTagsExpressionMutationA95KPromoterCAGAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
ER-GA
Plasmid#209871PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorInsertddGFP A
UseTagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
Plasmid#104394PurposeYeast MCP-GFP expressionDepositorInsertMCP-NLS-2xyeGFP
UseTagsExpressionBacterial and YeastMutationMCP fused to NLS(SV40) and 2x yeast codon optimiz…PromoterCYC1Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (Neo)
Plasmid#89180PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
BE4
Plasmid#100802PurposeExpresses BE4 in mammalian cellsDepositorInsertBE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgBFP
Plasmid#192478PurposeAll-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3DepositorInsertSpCas9
UseTagsT2A iRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
V5-GFP-APEX2-NES_pLX208
Plasmid#202018Purposeexpresses GFP-APEX2 in the mammalian cytosol, lentiviral vectorDepositorInsertV5-GFP-APEX2-NES
UseLentiviralTagsGFP and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1_Puro
Plasmid#125594PurposeLentiviral expression of sgRNA with GFP and puromycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDonor_ShCAST_kanR
Plasmid#127924PurposeKanamycin resistance donor for ShCASTDepositorInsertKanR, R6K origin of replication
UseTagsExpressionMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
mIL12-N1
Plasmid#123139PurposeMammalian expression of murine cytokine IL-12 under CMV promoterDepositorInsertMurine IL12 tandem p40 p35
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
UseTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_mGFP-FGFR3
Plasmid#191752PurposeExpression of N-terminally mGFP labelled wildtype FGFR3 (isoform IIIc)DepositorInsertmGFP-FGFR3IIIc
UseTagsmGFPExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gβ-2A-cpV-Gγ2-IRES-Gαi3-mTq2
Plasmid#69625PurposeA FRET sensor for Galphai3 activation, encoded on a single plasmid.DepositorInsertsGbeta-2A-cpV-Ggamma2
Galphai3-mTurquoise2
UseTagsciruclar permutated Venus (cp173V) and mTurquoise…ExpressionMammalianMutationPromoterCMV and CMV (IRES)Available sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Hs.cargo(Cre)
Plasmid#174863PurposeExpresses human Peg10 cargoRNA encoding Cre recombinaseDepositorInsertCre flanked by HsPeg10 UTRs
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2Cre
Plasmid#82415PurposeLentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning siteDepositorInsertCre Recombinase
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationMutated BsmBI cut sitePromoterEFS (P2A)Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
MYC_pLX307
Plasmid#98363PurposeLentiviral expression of MYCDepositorInsertMYC (MYC Human)
UseLentiviralTagsNAExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
GAL4BD-VP16/pDON207
Plasmid#201230PurposeGAL4-VP16 transcription factorDepositorInsertGAL4BD-VP16
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-NFE2L2-Puro
Plasmid#181919PurposeLentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.DepositorInsertNFE2L2 (NFE2L2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-C1
Plasmid#58186DepositorTypeEmpty backboneUseTagsSNAP tagExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL-CRISPR.SFFV.GFP
Plasmid#57827PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
CaV1.2/pcDNA3.1~ Human
Plasmid#140576PurposeExpression in mammallian cells or oocytesDepositorInserthuman CaV1.2 (CACNA1C Human)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tag5Amyc-GSK3b WT
Plasmid#16260DepositorInsertGSK3 beta (GSK3B Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a-alpha-synuclein
Plasmid#51486PurposeInducible expression of human alpha-synuclein in E.coliDepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 31, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYTK001
Plasmid#65108PurposePart Plasmid Entry Vector to be used in the Dueber YTK system.DepositorInsertnone
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLEX_305-N-dTAG
Plasmid#91797PurposeLentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG systemDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsFKBP F36VExpressionMammalianMutationPromoterhPGKAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-IRES-Neo
Plasmid#28019DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDESTmycIGF2BP3
Plasmid#19879DepositorInsertInsulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)
UseTagsMYCExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_pGK_mCherry
Plasmid#79124PurposeLentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors (no IRES)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcVpx.myc
Plasmid#213388PurposeGenerate lentiviral vector which increases gene delivery into primary human myeloid cellsDepositorInsertSIVmac vpx (vpx Simian Immunodeficiency Virus)
UseLentiviralTagsmyc-6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLOX-gfp-iresTK
Plasmid#12243DepositorInsertGFP, HSV1-TK
UseCre/Lox and LentiviralTagsExpressionMammalianMutationLoxP in 3' LTR.PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCBC-DT1T2
Plasmid#50590PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT1, U626t plus, U629p, T2
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-WT-VWF
Plasmid#124794PurposeExpresses multimeric human VWF with C-terminal c-Myc tagDepositorInsertVWF (VWF Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pN7HELIX
Plasmid#190662PurposeExpresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRESDepositorInserthuman codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
UseTagsExpressionMammalianMutationnAniI = K227M, F80K, L232KPromoterCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABe-HA-ER-IPpoI
Plasmid#32565DepositorInsertI-PpoI
UseRetroviralTagsER and HAExpressionMammalianMutationnonePromoterAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP_CD47_LU
Plasmid#65474PurposeGFP tagged CD47 with its own long 3'UTRDepositorInsertsSignal peptide of CD47
GFP
CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only