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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 34993 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCCL-cppt-PGK-WPRE
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Backbone manufacturerMalin Parmar
- Backbone size w/o insert (bp) 7041
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHomeobox protein engrailed-1
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Alt nameEn1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1206
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GenBank IDNT_078297.6
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Entrez GeneEn1 (a.k.a. En-1, Mo-en.1, engrailed-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer ACAACCCTGCGATCCTACTCAT
- 3′ sequencing primer GGGTTGCTGTGAGTCAGTCTTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositing lab is aware of the discrepancies between the Addgene quality control sequence and the depositor's sequence and affirm that the mutations do not alter the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
En1 was a gift from Malin Parmar (Addgene plasmid # 34993 ; http://n2t.net/addgene:34993 ; RRID:Addgene_34993) -
For your References section:
Direct conversion of human fibroblasts to dopaminergic neurons. Pfisterer U, Kirkeby A, Torper O, Wood J, Nelander J, Dufour A, Bjorklund A, Lindvall O, Jakobsson J, Parmar M. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10343-8. Epub 2011 Jun 6. 10.1073/pnas.1105135108 PubMed 21646515