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Plasmid#39552DepositorInserthelicase (Hel25E Fly)
UseTagsExpressionMutationPromoterAvailable sinceJan. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.0
Plasmid#190795PurposePRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning processDepositorInsertPRE-Hsp70Bb-Cas9-T2A-eGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCh-76
Plasmid#92288PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cellsDepositorInsertdGW182 CED (gw Fly)
UseTagsNHAExpressionInsectMutationdGW182 CED: aa 940-1215PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Renilla luciferase-Pol III
Plasmid#37380DepositorInsertPolIII-Renilla control reporter (RpIII128 Fly)
UseLuciferaseTagsExpressionMammalianMutationPromoterRNA PolIII 128 subunit (RpIII128)Available sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.2
Plasmid#190796PurposegypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.DepositorInsertgypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.3
Plasmid#190797Purposeaka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final productDepositorInsert2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pUAST-HA, Pbl-A
Plasmid#69792PurposePebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble - Drosophila guanine nucleotide exchange factor
UseP element-based puast vector for gal4-regulated e…TagsHA tagExpressionInsectMutationEncodes Pbl (NP_729306.1) lacking last 9 amino ac…Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmAGO2-RC_B
Plasmid#145980PurposeInsect Expression of DmAGO2-RCDepositorInsertDmAGO2-RC (AGO2 Fly)
UseTagsExpressionInsectMutationA deletion of AA 51-56 and an insertion of 23 AA …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A DH domain
Plasmid#69794PurposePebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble DH domain
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationContains only the Pebble DH domainPromoterhsp70Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO2-RC_B
Plasmid#145981PurposeInsect Expression of DmAGO2-RCDepositorInsertDmAGO2-RC (AGO2 Fly)
UseTagsExpressionInsectMutationA deletion of AA 51-56 and an insertion of 23 AA …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCh-623
Plasmid#92266PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cellsDepositorInsertdGW182 6W6A (gw Fly)
UseTagsNHAExpressionInsectMutationThe following point mutations were introduced W23…PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-695
Plasmid#92263PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cellsDepositorInsertdGW182 PAM2 mut (gw Fly)
UseTagsNHAExpressionInsectMutationThe following point mutations were introduced: EF…PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A C-Term
Plasmid#69795PurposePebble isoform A, C-Term, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble C-Term domain (Drosophila guanine nucleotide exchange factor)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationJust the C-Term domain of Pebble (pbl)Promoterhsp70 promoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMCh-646
Plasmid#92289PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cellsDepositorInsertdGW182 CED 7W7A (gw Fly)
UseTagsNHAExpressionInsectMutationdGW182 CED: aa 940-1215 with the following point …PromoterAc5Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A PH+DH domains
Plasmid#69793PurposePebble isoform A, PH and DH domains, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble DH +PH domains (pbl Fly)
UseP element-based puast vector for gal4-regulated e…TagsHA tagExpressionInsectMutationPbl-A (NP_729306.1) amino acids 385-647.Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCoHygro L16_shRNA of spliced Copia
Plasmid#71389Purposeknock down expression of spliced Copia in Drosophila melanogasterDepositorInsertCopia
UseTagsExpressionInsectMutationPromoterU6Available sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -