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Showing: 251 - 300 of 1663 results

  2. pCIBN-tagBFP-hTRF2

    Plasmid
    #103803
    Purpose
    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-tagBFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hTRF2: deletion of amino acids 1-42 and 476 compa…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103803-plasmid-map-sequence-id-199226

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  7. CIBN-rTetR

    Plasmid
    #183919
    Purpose
    Optogenetic CIBN localizer domain coupled to reverse Tet repressor; binds tetO sites upon doxycycline addition; CIBN is bound by PHR upon blue light exposure
    Depositor
    Karsten Rippe
    Article
    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22
    Insert
    CIBN-rTetR (CIB1 Synthetic, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    June 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183919-plasmid-map-sequence-id-364132

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  11. pCIBN-EYFP-hTRF2

    Plasmid
    #103804
    Purpose
    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-EYFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hTRF2: deletion of amino acids 1-42 and 476 compa…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103804-plasmid-map-sequence-id-199232

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  19. pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)

    Plasmid
    #182395
    Purpose
    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396
    Inserts
    VNp-LZ-VenusN154
    VNp-LZ-VenusC155
    Use
    Tags
    Vesicle Nucleating peptide (VNp) & Leucine Zi…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    182395-plasmid-map-sequence-id-364922

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  20. pPHR-mCherry-NLS-VP16

    Plasmid
    #103821
    Purpose
    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    PHR-mCherry-VP16 (CRY2 Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    VP16: G126T (silent, G42G)
    Promoter
    CMV
    Available since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    103821-plasmid-map-sequence-id-203431

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  21. pPHR-iRFP713-NLS-VP16

    Plasmid
    #103823
    Purpose
    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    PHR-iRFP713-VP16 (CRY2 Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    VP16: G126T (silent, G42G)
    Promoter
    CMV
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    103823-plasmid-map-sequence-id-199372

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  23. pCIBN-TetR-tagRFP-T

    Plasmid
    #103809
    Purpose
    'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-TetR-tagRFP-T (CIB1, tetR E. coli, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    TetR: A4G (M2V)
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103809-plasmid-map-sequence-id-199261

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  42. pCIBN-hTRF2-tagRFP-T

    Plasmid
    #103812
    Purpose
    BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-hTRF2-tagRFP-T (CIB1, TERF2 Human, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hTRF2: deletion of amino acids 1-42 and 476 compa…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103812-plasmid-map-sequence-id-199277

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  43. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

    Plasmid
    #182424
    Purpose
    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396
    Inserts
    VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
    VNp-LZ
    Use
    Tags
    Vesicle Nucleating peptide (VNp), and Vesicle Nuc…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    182424-plasmid-map-sequence-id-360472

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  45. pCIBN-tagBFP-hNCL

    Plasmid
    #103805
    Purpose
    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-tagBFP-hNCL (CIB1, NCL Human, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103805-plasmid-map-sequence-id-199237

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  47. pCIBN-LacI

    Plasmid
    #103814
    Purpose
    BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-LacI (CIB1, lacI E. coli, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    LacI: C19T (silent, L7L), T264C (silent, A88A), C…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103814-plasmid-map-sequence-id-199324

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  48. pCIBN-EYFP-hNCL

    Plasmid
    #103806
    Purpose
    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-EYFP-hNCL (CIB1, NCL Human, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103806-plasmid-map-sequence-id-199240

    Enlarge

Showing: 251 - 300 of 1663 results