pLV 4xSTAR-mTagBFP2-blast
(Plasmid
#125239)
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PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 125239 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size w/o insert (bp) 6256
- Total vector size (bp) 8384
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namestem cell Ascl2 reporter, 4 repeats
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Alt name4x STAR
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)350
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Tag
/ Fusion Protein
- mTagBFP2 (codon optimized)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV 4xSTAR-mTagBFP2-blast was a gift from Hugo Snippert (Addgene plasmid # 125239 ; http://n2t.net/addgene:125239 ; RRID:Addgene_125239) -
For your References section:
Specific Labeling of Stem Cell Activity in Human Colorectal Organoids Using an ASCL2-Responsive Minigene. Oost KC, van Voorthuijsen L, Fumagalli A, Lindeboom RGH, Sprangers J, Omerzu M, Rodriguez-Colman MJ, Heinz MC, Verlaan-Klink I, Maurice MM, Burgering BMT, van Rheenen J, Vermeulen M, Snippert HJG. Cell Rep. 2018 Feb 6;22(6):1600-1614. doi: 10.1016/j.celrep.2018.01.033. 10.1016/j.celrep.2018.01.033 PubMed 29425513
Map uploaded by the depositor.
