Addgene

Showing: 101 - 120 of 2163 results

Results per page:

Sort results by:

FUGW-VMAT2-pHluorin

Plasmid

#207468

Purpose

Lentiviral expression of VMAT2-pHluorin

Depositor

Robert Edwards

Article

Silm et al Neuron. 2019 May 22;102(4):786-800.e5. d

Insert

VMAT2 (Slc18a2 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-CMV-Galpha-i1 ONE-GO

Plasmid

#189735

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i1 ONE-GO
GNAI1 (Gnai1 Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mEGFP-CaMKIIa (T286D)

Plasmid

#127391

Purpose

Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

changed Threonine 286 to Aspartic acid

Promoter

pCAG

Available since

Jan. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK286 (mAID-NeoR)

Plasmid

#72824

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Neo

Use

CRISPR

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
iRFP-PH-PLCdelta1

Plasmid

#66841

Purpose

expression of near-infrared PI(4,5)P2 biosensor

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

PH-PLCdelta1

Use

Tags

iRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT-HA-rM3D(Gs)

Plasmid

#45549

Purpose

Expresses rM3D (Gs) neuronal activator

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

rM3D (Gs)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs GFP-LC3-RFP

Plasmid

#117413

Purpose

Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

Depositor

Noboru Mizushima

Article

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
HSV-DYN-hM4Di

Plasmid

#53327

Purpose

Dynorphin promoter driving hM4Di

Depositor

John Neumaier

Article

Ferguson et al Nat Neurosci. 2011 Jan;14(1):22-4. doi:

Insert

hM4Di (Pdyn Rat)

Use

Hsv

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Rat dynorphin promoter ( -1858 to +135)

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Lysosomes-20

Plasmid

#55073

Purpose

Localization: Lysosome Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

LAMP1

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-His6-ERK2-MEK1_R4F_coexpression

Plasmid

#39212

Depositor

Melanie Cobb

Article

Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

Inserts

Mek1 R4F (MAP2K1 Human)
Erk2 (Mapk1 Rat)

Use

Tags

His6 and RBS

Expression

Bacterial

Mutation

MEK1(R4F) = S218E, S222D and deletion of AA32-51

Promoter

T7 and T7 via RBS

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1 F-tractin-EGFP

Plasmid

#58473

Purpose

Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

F-tractin (Itpka Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-mTOR

Plasmid

#1861

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

mTOR (Mtor Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE Tubb3-GFP KI

Plasmid

#131497

Purpose

Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CaVα2δ1

Plasmid

#26575

Depositor

Diane Lipscombe

Article

Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

Insert

Cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW-VGLUT1-pHluorin

Plasmid

#207470

Purpose

Lentiviral expression of VGLUT1-pHluorin

Depositor

Robert Edwards

Insert

VGLUT1 (Slc17a7 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV2-SEP-GluA1 (M1)

Plasmid

#64942

Purpose

mammalian expression of rat GluA1

Depositor

Jeremy Henley

Article

Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

Insert

GluA1 (Gria1 Rat)

Use

Tags

SEP (superecliptic pHluorin)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti sfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164478

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

mCherry and superfolder GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-C1-CAMKIIbeta

Plasmid

#21227

Depositor

Tobias Meyer

Article

Shen et al Neuron. 1998 Sep . 21(3):593-606.

Insert

CAMKIIbeta (Camk2b Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Lab plasmid KS 0002.

Promoter

Available since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
TrkC-GFP

Plasmid

#24094

Depositor

Moses Chao

Insert

TrkC (Ntrk3 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
pLV-Ftractin-mCherry

Plasmid

#85131

Purpose

lentiviral expression of an F-actin live-cell marker (mCherry)

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

EF1a

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge