Addgene

Showing: 11921 - 11940 of 18816 results

Results per page:

Sort results by:

pDONR221_SLC4A9

Plasmid

#132216

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A9 (SLC4A9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGAL4-DBD-BHLHE41wt-IDR

Plasmid

#145229

Purpose

Expresses fusion of GAL4 DNA-binding domain and BHLHE41wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

BHLHE41wt-IDR (BHLHE41 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
px458_2A_GFP_sgRNA_CELF2

Plasmid

#106102

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting CELF2 (CELF2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LRG/Foxa1 e2.4

Plasmid

#105511

Purpose

Lentiviral expression of Foxa1 DNA-binding domain (exon 2) targeting sgRNA

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Foxa1 (sgRNA, e2.4)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_BRD003-sgRBFOX1-2098

Plasmid

#115452

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgRBFOX1-2098 (RBFOX1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_BRD003-sgRBFOX1-2083

Plasmid

#115451

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgRBFOX1-2083 (RBFOX1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Flag MFF iso1 S172A

Plasmid

#74391

Purpose

Flag tagged human S172A MFF isoform 1 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

delta exon 1 (1-26) and Ser 172 Ala.

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pGAL4-DBD-HOXA13wt-IDR

Plasmid

#145240

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13wt-IDR (HOXA13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP

Plasmid

#69050

Purpose

lentiviral expression of IKZF3 with Q147H mutation and FLAG tag

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF3 (IKZF3 Human)

Use

Lentiviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

Q147H

Promoter

CAG

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Basic-Fascin-Promoter (-402-0)

Plasmid

#89823

Purpose

Promoter luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

SV40

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Mouse GFP-PRMT7 LDIG(69-72)AAAA

Plasmid

#65044

Purpose

Expresses Catalytically inactive GFP-PRMT7 LDIG(69-72)AAAA mutant

Depositor

Steven Clarke

Article

Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

Insert

PRMT7 (Prmt7 Mouse)

Use

Bacterial propagation

Tags

GFP

Expression

Mammalian

Mutation

L69A D70A I71A G72A

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pECE beta1 R760A

Plasmid

#50511

Depositor

Kenneth Yamada

Article

Pankov et al J Biol Chem. 2003 May 16;278(20):18671-8

Insert

integrin beta1 (ITGB1 Human)

Use

Tags

Expression

Mammalian

Mutation

R760A

Promoter

SV40

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
CMV-138_HNF4A2

Plasmid

#31091

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 . 2():210.

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

splice variant 2 of HNF4A, deletion of CMV promo…

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-DICER-Prom upstream Ebox Mut

Plasmid

#25852

Depositor

David E. Fisher

Article

Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

Insert

DICER-Promoter upstream Ebox Mut (DICER1 gene ID 23405, Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-FH-AGO2-S828A

Plasmid

#91982

Purpose

Expresses FLAG-HA-AGO2 (S828A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828A

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BMR1_02g02691-bio-His

Plasmid

#108042

Purpose

Expresses enzymatically monobiotinylated full-length BMR1_02g02691 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

Insert

BMR1_02g02691

Use

Tags

ratCD4d3+4,biotinylation sequence, 6xHis (C termi…

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BMR1_03g01540-bio-His

Plasmid

#108043

Purpose

Expresses enzymatically monobiotinylated full-length BMR1_03g01540 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

Insert

BMR1_03g01540

Use

Tags

ratCD4d3+4,biotinylation sequence, 6xHis (C termi…

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 MLK1 E179K

Plasmid

#60541

Purpose

Donor vector for human MLK1 E179K mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

E179K

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-DICER-Prom downstream Ebox Mut

Plasmid

#25853

Depositor

David E. Fisher

Article

Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

Insert

DICER-Prom downstream Ebox Mut (DICER1 gene ID 23405, Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-158/+19

Plasmid

#35541

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

none

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge