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A library of recombinant Babesia microti cell surface and secreted proteins for diagnostics discovery and reverse vaccinology.

Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
Int J Parasitol. 2019 Feb;49(2):115-125. doi: 10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
107665BMR1_01g00210-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g00210 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
107666BMR1_03g04695-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g04695 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
107667BMR1_01g03335-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g03335 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
107668BMR1_02g04181-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_02g04181 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
107669BMR1_01g03280-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g03280 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
107670BMR1_04g07915-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_04g07915 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
107671BMR1_01g02975-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g02975 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
107672BMR1_03g01156-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g01156 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
107673BMR1_04g08630-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_04g08630 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
107674BMR1_03g02090-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g02090 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
107675BMR1_01g00985-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g00985 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108000BMR1_03g04335-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g04335 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108001BMR1_03g00437-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g00437 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108002BMR1_04g06950-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_04g06950 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108003BMR1_01g01210-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g01210 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108004BMR1_02g04260-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_02g04260 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108005BMR1_03g00785-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g00785 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108006BMR1_04g07810-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_04g07810 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108007BMR1_04g09190-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_04g09190 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108008BMR1_02g04275-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_02g04275 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108009BMR1_01g00435-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g00435 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108010BMR1_01g01875-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g01875 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108011BMR1_01g00425-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g00425 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108012BMR1_04g05790-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_04g05790 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108013BMR1_01g00430-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g00430 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108014BMR1_03g01685-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g01685 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108015BMR1_03g03430-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g03430 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108016BMR1_04g07535-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_04g07535 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108017BMR1_01g00095-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g00095 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108019BMR1_01g01510-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g01510 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108020BMR1_01g03475-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g03475 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108021BMR1_01g00945-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g00945 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108022BMR1_03g02875-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g02875 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108023BMR1_03g00790-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g00790 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108024BMR1_03g04855-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g04855 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108025BMR1_03g00690-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g00690 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108026BMR1_03g04550-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g04550 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108027BMR1_04g05295-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_04g05295 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108028BMR1_02g00320-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_02g00320 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108029BMR1_02g00615-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_02g00615 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108030BMR1_01g02875-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g02875 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108031BMR1_02g00700-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_02g00700 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108032BMR1_03g03090-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g03090 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108033BMR1_03g00020-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g00020 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108034BMR1_04g09435-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_04g09435 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108035BMR1_04g08470-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_04g08470 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108036BMR1_03g02515-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g02515 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108037BmR1_04g07470-bio-HisExpresses enzymatically monobiotinylated full-length BmR1_04g07470 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108038BMR1_01g02876-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_01g02876 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108040BMR1_03g02385-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g02385 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108041BMR1_02g04075-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_02g04075 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108042BMR1_02g02691-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_02g02691 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108043BMR1_03g01540-bio-HisExpresses enzymatically monobiotinylated full-length BMR1_03g01540 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
108116BMR1_01g02031-bio-His[Edit] Expresses enzymatically monobiotinylated full-length BMR1_01g02031 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag

Antibodies from Article