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pGAMA-empty

Plasmid

#74755

Purpose

Mammalian lentiviral vector

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Plasmid

#159798

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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PB-TET-mSox2

Plasmid

#20908

Purpose

piggyBac vector with tet inducible mSox2

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Mar 1. ():.

Insert

Sox2 (Sox2 Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-CERP2-MG-IRES2-mTagBFP2

Plasmid

#175509

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G-mCMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Plasmid

#159803

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-KPNA2 zeo

Plasmid

#192330

Purpose

Lentiviral expression vector for an inducible 3xFLAG-KPNA2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNA2 (KPNA2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV NS5

Plasmid

#145348

Purpose

Cell-free/ mammalian expression vector for Zika virus NS5 protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS5

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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Puro-iNotch1IC

Plasmid

#75338

Purpose

doxycycline inducible Notch1 intracellular domain gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

Notch1 Intracellular domain (Notch1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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MiniCoopR 2xU6:gRNA, mitfa:Cas9

Plasmid

#118844

Purpose

Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Type

Empty backbone

Use

CRISPR; Tol2

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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PB-hEF1A-MG-U6-sgRNA

Plasmid

#175505

Purpose

All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

hEF1a, U6

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBH-UAS-secA5-YFP

Plasmid

#32359

Purpose

Live imaging of apoptotic cells in zebrafish

Depositor

Randall Peterson

Article

van Ham et al FASEB J. 2010 Nov;24(11):4336-42. Epub 2

Insert

secreted human annexin 5 (ANXA5 Human)

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

UAS

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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MAC_C_LMTK2

Plasmid

#187752

Purpose

MAC-tagged gene expression of human LMTK2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

LMTK2 (LMTK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-p38KTRClover

Plasmid

#59142

Purpose

ORF encoding p38 KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-ERP2-MG-IRES2-mNeonGreen

Plasmid

#175506

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G-mCMV

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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p5e irg1

Plasmid

#188698

Purpose

Gateway promoter element for the zebrafish irg1 gene

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

irg1 (acod1 Zebrafish)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

irg1

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE-SHH

Plasmid

#134350

Purpose

Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

Depositor

Lorenz Studer

Article

Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

Insert

SHH (SHH Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:EGFP

Plasmid

#118850

Purpose

Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

mitfa: EGFP (mitfa Zebrafish, Synthetic)

Use

Zebrafish expression, tol6

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

Plasmid

#159799

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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#1.4 post-R-SynapShot-Lyn-miRFP670

Plasmid

#214718

Purpose

Expresses red #1.4 post-SynapShot and Lyn-miRFP670

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#1.4 post-R-SynapShot

Use

Tags

Lyn-miRFP670

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMH-CMV-GFP

Plasmid

#133017

Purpose

(Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under CMV promoter.

Depositor

Michael Huen

Article

Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

Type

Empty backbone

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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