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Plasmid#115798Purposelentiviral, trimethoprim (TMP) regulated YAP promoter activity reporterDepositorInsertYAP responsive promoter (YAP1 Human)
UseLentiviralTagsDsRED-DHFRExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-PcKRS
Plasmid#140022PurposePlasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integrationDepositorInsertPcKRS (pylS Methanosarcina bakeri)
UseTagsFLAGExpressionMammalianMutationM241F, A267S, Y271C, L274MPromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA
Plasmid#139192PurposeExpresses C-terminal HA tagged wt human Mfn2 in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags3HAExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
spisPink chromoprotein
Plasmid#117839PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coliDepositorInsertpromoter, RBS, spisPink
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF1253
Plasmid#142764PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFOS WT-GL3
Plasmid#11983PurposeReporter gene with mouse c-fos promoter. Induced by serum and many growth factors in quiescent cells.DepositorInsertc-fos promoter (Fos Mouse)
UseTagsLucExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2_mSTING_scrambled_gRNA_1
Plasmid#196627PurposeScrambled control 1 for STING knock-out in murine cells.DepositorInsertmSTING scrambled gRNA 1
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-Puro_hCOL4A6-Myc-His
Plasmid#198087PurposeExpress hCOL4A6 in mammalian cellsDepositorInsertCOL4A6 (COL4A6 Human)
UseTagsTEV-Myc-HisExpressionMammalianMutationPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2 HRS-RFP
Plasmid#29685DepositorInserthepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)
UseTagsRFPExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
TFORF2482
Plasmid#142103PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMXD3 (MXD3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDest-311 (attR4-attR3)
Plasmid#161896PurposeMultisite Gateway (attR4-attR3) vector for mammalian protein expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-GtACR1
Plasmid#85464PurposeNatural anion conducting Channelrhodopsin with cyan absorption. Closing kinetics 140ms. High conductivity. Codon optimized for mammalian expression (human/mouse)DepositorInsertSynthetic construct ACR1 gene
UseTagsmCherryExpressionMammalianMutationPromoterCMV (+enhancer)Available sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
SFFV-AID-NTERM-GFP
Plasmid#185763PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, AIDExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-sfCherry11x4-ß-actin
Plasmid#70220PurposeExpresses sfCherry11x4-ß-actin in mammalian cellsDepositorInsertsfCherry11x4-actin (ACTB Human)
UseTagssfCherry11x4ExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHyo094 (eMutaT7)
Plasmid#173124PurposeExpresses eMutaT7 for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertpmCDA1-T7RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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mEos3.2-N1
Plasmid#54525PurposeLocalization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580DepositorTypeEmpty backboneUseTagsmEos3.2ExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-Beclin 1
Plasmid#86748PurposeN-terminal HA-tagged protein expression in mammalian cellsDepositorInserthuman Beclin 1 (BECN1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRES2_EGFP_KCNQ1
Plasmid#173161PurposeFor mammalian expression of KCNQ1 and creating variantsDepositorInsertKCNQ1 (KCNQ1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-BirA*-pDEST-N-pcDNA5/FRT/TO
Plasmid#118375PurposeExpression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.DepositorTypeEmpty backboneUseDestinationTagsHA-BirA*ExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only