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Dual stop codon suppression in mammalian cells with genomically integrated genetic code expansion machinery.

Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, Landreh M, Elsasser SJ
Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
140018pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAGPlasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration
140019pAS_4xEcoLeuT(CUA)_AnapRSPlasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of azido-phenylalanine; for transient or stable piggyBac-mediated integration
140020pAS_4xMma PylT_FLAG-AbKRS-chIPYEPlasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration
140021pAS_4xMma PylT_FLAG-AcKRSPlasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration
140022pAS_4xMma PylT_FLAG-PcKRSPlasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration
174891pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAGamber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette
174892pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAGamber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette
174894pAS_4xEcoLeuT(CUA)_EF1_AnapRSAnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration
174895pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAAamber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette
174896pAS_4xMmaPylT(UUA)_EF1_MmaPylRSMma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration
174897pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAAochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette
174898pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGAopal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette
174899pAS_4xMmaPylT(UCA)_EF1_MmaPylRSMma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

Antibodies from Article