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Plasmid#86774PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertimmunoproteasome beta subunit 2 (MECL1) (PSMB10 Human)
UseLentiviralTagsflagExpressionMammalianMutationPromoterunknownAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_260d
Plasmid#91126PurposeTransformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterPvUbi2Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYES2-HTH
Plasmid#86470PurposeYeast expression vector containing HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoterDepositorTypeEmpty backboneUseTagsHIS10-TEV-HAExpressionYeastMutationPromoterGAL1Available sinceJan. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBabe puro IkB alpha M
Plasmid#12332DepositorInsertIkB alpha M (Nfkbia Mouse)
UseRetroviralTagsExpressionMammalianMutationDominant-negative mutant. Many signal transductio…PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-hPKM2
Plasmid#44242DepositorInsertPyruvate Kinase M2 (PKM Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF1252
Plasmid#143778PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST12.2 DYRK2 WT
Plasmid#20005DepositorInsertDYRK2 (DYRK2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
PB513B-1/TRE-hHNF1β-hHNF4α-hHNF6-EF1α-Bla
Plasmid#199551PurposePiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1β-hHNF4α-hHNF6) under control of the TRE/PCMVmin promoterDepositorUsePiggybac transposon vectorTagsExpressionMammalianMutationPromoterTRE+CMVmin promoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)
Plasmid#191141PurposePositive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codonDepositorInsertmCherry:s70587-W:EGFP
UseTagsExpressionMammalianMutationUAG>UGG (stop>Trp)PromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING-342STOP
Plasmid#102588PurposeLentivector to express STING delta 342 and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationdelta 342PromoterSFFVAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSkylan-NS-N1
Plasmid#86785Purposefor fusion of gene of interest with a monomeric reversibly photoswitchable fluorescent protein, Skylan-NSDepositorTypeEmpty backboneUseTagsSkylan-NSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
FCM101
Plasmid#194298PurposeE. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6DepositorInsertFCM101
UseTagsHis tag, FLAG tagExpressionBacterialMutationPromoterT7Available sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRT006.3 L1 dual-luciferase AI retrotransposition reporter
Plasmid#213031PurposeBi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integrationDepositorInsertTet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
UseLuciferaseTagsFirefly luciferase antisense intron (3' UTR)ExpressionMammalianMutationPromoterpTRE bidirectional tet-onAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMJA219= pcDNA3.1V5-completeTRABV-CMVp
Plasmid#116878Purposemammalian expression vector containing TCR sequencesDepositorUseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPLV_C1
Plasmid#186415PurposeEndogenous murine RNase inhibitor production for cell-free lysateDepositorInsertMurine RNase Inhibitor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cas12f-GE ver4.1
Plasmid#176544PurposeExpresses Cas12f-GE in mammalian cellsDepositorInsertsCas12f-GE ver4.1
Cas12f-GE ver4.1
UseTagsExpressionMammalianMutationPromoterU6 and chicken β-actinAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV5S-F30-2xdBroccoli
Plasmid#66845PurposeExpresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cellsDepositorInsertTwo dimeric Broccoli aptamers in the F30 scaffold
UseTags5S rRNAExpressionMammalianMutationPromoter5SAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF0924
Plasmid#141757PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR2F2 (NR2F2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-EGFPL10a
Plasmid#98747PurposeCre-dependently expresses the fusion protein EGFPL10aDepositorHas ServiceAAV Retrograde and AAV5InsertEGFPL10a
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSaGuide
Plasmid#64710PurposeCloning and expression of Staphylococcus aureus guide RNADepositorInsertStaphylococcus aureus guide RNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only