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Plasmid#102245PurposeLentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.DepositorInsertdCas9-VPR-mCherry
UseCRISPR and LentiviralTagsNLS-VPR-mCherryExpressionMammalianMutationPromoterCAGAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTAGBFP2 Peroxisome visual barcode
Plasmid#158668Purposetagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WCDepositorInsertmTAGBFP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shBRCA1 #1
Plasmid#44594DepositorInsertBRCA1 (Brca1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hSTING (R232)
Plasmid#214152PurposeTransient expression of STING R232DepositorInsertSTING (STING1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CTNNB1 in pENTR
Plasmid#216617Purposegateway cloning donor vector containing CTNNB1DepositorInsertCTNNB1 (CTNNB1 Human)
UseGateway entry plasmidTagsExpressionMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV5S-F30-2xdBroccoli
Plasmid#66845PurposeExpresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cellsDepositorInsertTwo dimeric Broccoli aptamers in the F30 scaffold
UseTags5S rRNAExpressionMammalianMutationPromoter5SAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGrDL_SP
Plasmid#83204Purposereceives miRNA target sequences for testing using the dual luciferase assay systemDepositorInsertSalI/PstI cloning sites
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS-H2B-citrine
Plasmid#53752PurposeContains fusion of human Histone H2B to citrine.DepositorInserthistone H2B (H2BC21 Human)
UseTagscitrineExpressionMammalianMutationPromotersimian CMV IE94Available sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgHK2_2
Plasmid#163461Purposelentiviral vector expressing Cas9 and an sgRNA targeting HK2DepositorInsertsgRNA 2 targeting HK2 (HK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
SuExp His-Myc-hPLD3
Plasmid#173851PurposeProtein expression plasmid for recombinant His-Myc tag human PLD3DepositorInsertPLD3 (PLD3 Human)
UseTags6xHis, Myc, Factor X cleavableExpressionMammalianMutationintracellular and transmembrane domain truncated,…PromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3β-FLAG-CBP-HA
Plasmid#32908DepositorInsertCBP (Crebbp Mouse)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-B19L
Plasmid#59922PurposeExpression vector for Rabies virus SAD B19 RNA dependent RNA polymerase geneDepositorInsertRabies virus RNA dependent RNA polymerase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RET-V5/HIS
Plasmid#205606PurposeExpression of human RET receptor tyrosine kinase in mammalian cellsDepositorInsertRET receptor tyrosine kinase (RET Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUSE-RapR-Src(KD)-myc
Plasmid#25934DepositorInsertRapR-Src (Fkbp1a Mouse)
UseTagsExpressionMammalianMutationThis pUSE-RapR-Src(KD)-myc plasmid contains the k…PromoterAvailable sinceOct. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
DT12A
Plasmid#80416PurposeExpresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-hPKM2
Plasmid#44242DepositorInsertPyruvate Kinase M2 (PKM Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB c Rosella I
Plasmid#71245Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.DepositorInsertRosella
UseTagsExpressionYeastMutationPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
peSpCas9(1.1)-2×sgRNA (IFT88, donor)
Plasmid#80769PurposeExpresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).DepositorInsertIFT88 gRNA#1 (IFT88 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMA4008
Plasmid#70109PurposemtDNA destruction/production of rho-0 cellsDepositorInsertsEGFP
Herpes simplex virus UL12.5M185
UseTagsnoneExpressionMammalianMutationN-terminal deletion of UL12.5PromoterCMV and RSVAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-puro-hRAF1-shRNA-1
Plasmid#185371PurposeFor tetracycline-inducible mammalian expression of shRNA: CATGAGTATTTAGAGGAAGTA that targets human RAF1DepositorInsertRAF1 (RAF1 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only