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pT7-EGFP-C1-HsNXF-1_K

Plasmid

#146740

Purpose

Mammalian Expression of HsNxf-1

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

HsNxf-1 (NXF1 Human)

Use

Tags

Expression

Mammalian

Mutation

S144G relative to NM_006362.4

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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HK2 gRNA (BRDN0001162212)

Plasmid

#76310

Purpose

3rd generation lentiviral gRNA plasmid targeting human HK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HK2 (HK2, HOOK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_dSV40-H2B-GFP

Plasmid

#67928

Purpose

Expresses H2B-GFP in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

H2B-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab9a

Plasmid

#78592

Purpose

Express mCherry-Rab9a in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

Rab9a (RAB9A Human)

Use

Tags

mCherry2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAP4K2

Plasmid

#23644

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP4K2 (MAP4K2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pEMS1113

Plasmid

#29042

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple51 (DBH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72667

Purpose

Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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p663-UBC-V5-CDK13_IDG-K

Plasmid

#135268

Purpose

Gateway destination clone of CDK13 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

V5-CDK13 (CDK13 Human)

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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PRPF19

Plasmid

#155847

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF19 (PRPF19 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJK1

Plasmid

#111722

Purpose

to express human BLM protein in yeast cells

Depositor

Ian Hickson

Article

Karow et al J Biol Chem. 1997 Dec 5;272(49):30611-4.

Insert

BLM (BLM Human)

Use

Tags

hexahistidine

Expression

Yeast

Mutation

a C-terminal hexahistidine tag was added, a yeast…

Promoter

GAL1

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_mSTING_scrambled_gRNA_1

Plasmid

#196627

Purpose

Scrambled control 1 for STING knock-out in murine cells.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING scrambled gRNA 1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-N2-Frankenbody-HA

Plasmid

#175292

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-HA frankenbody fused with mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-NEMOc

Plasmid

#189933

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-S100A11

Plasmid

#107201

Purpose

Expresses GFP-human S100A11 fusion protein for live cell imaging

Depositor

Ursula Rescher, Volker Gerke

Insert

S100A11 (S100A11 Human)

Use

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAW240

Plasmid

#170791

Purpose

P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution

Depositor

Chang Liu

Article

Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

Insert

p10B2-AT110

Use

Tags

Expression

Yeast

Mutation

Promoter

10B2 (P1 specific promoter)

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pUB-Cas9-@GL1

Plasmid

#86779

Purpose

Disruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9

Depositor

Andreas Weber

Article

Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

Insert

sgRNA against GLABROUS1

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Arabidopsis U6-26 promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-eGFP-Cre

Plasmid

#120219

Purpose

Expresses cre under the control of CamKIIa promotor

Depositor

Marie Carlén

Article

Ahrlund-Richter et al Nat Neurosci. 2019 Apr;22(4):657-668. do

Insert

cre

Use

AAV and Cre/Lox

Tags

EGFP

Expression

Mutation

Promoter

Available since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-Uricase

Plasmid

#182401

Purpose

Bacterial expression of Vesicle Nucleating peptide-uricase fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-uricase

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHsSR beta deltaN Flag

Plasmid

#13731

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRPRB Human)

Use

Tags

6xHis and FLAG

Expression

Bacterial

Mutation

expresses residues 59-271 of Homo sapiens SRβ wit…

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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GFAP-Cre

Plasmid

#24704

Depositor

Michael Sofroniew

Article

Bush et al Neuron. 1999 Jun . 23(2):297-308.

Insert

Cre

Use

Tags

Expression

Mammalian

Mutation

Promoter

GFAP (glial fibrillary acidic protein)

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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