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pLKO-shBRCA1 #2

Plasmid

#44595

Depositor

Eros Lazzerini Denchi

Article

Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

Insert

BRCA1 (Brca1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only
cfSGFP2-N

Plasmid

#37533

Depositor

Ikuo Wada

Article

Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

Insert

cysteine-free SGFP2-N

Use

Tags

Expression

Mammalian

Mutation

C48S and C70M mutations relative to SGFP2

Promoter

CMV

Available since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pSaGuide

Plasmid

#64710

Purpose

Cloning and expression of Staphylococcus aureus guide RNA

Depositor

Kiran Musunuru

Insert

Staphylococcus aureus guide RNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PML_RARA

Plasmid

#205897

Purpose

Express mEGFP-tagged fusion protein, PML_RARA from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PML_RARA (PML, RARA Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-pgk-puro

Plasmid

#85747

Purpose

tetracyline-inducible lentiviral vector with no insert for use in mammalian cells

Depositor

Emily Dykhuizen

Article

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Type

Empty backbone

Use

Lentiviral

Tags

none

Expression

Mutation

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pFl Strep-sumo-TEV-hAgo2

Plasmid

#136235

Purpose

Expresses human Argonaute-2 in insect cells

Depositor

Leemor Joshua-Tor

Article

Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

Insert

human Argonaute-2 (AGO2 Human)

Use

Tags

Strep Sumo

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_Puro

Plasmid

#167863

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSL0341 (pTarget)

Plasmid

#200906

Purpose

Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

pTarget

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-LMNB1

Plasmid

#207770

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-GFP

Plasmid

#129092

Purpose

KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Article

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

EGFP and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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6His-MBP-TEV-huLbCpf1

Plasmid

#90096

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Use

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLIB

Plasmid

#80610

Purpose

pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_Gag-EIF4E

Plasmid

#212657

Purpose

piggyBac vector to express an MLV Gag-EIF4E fusion protein

Depositor

Paul Blainey

Article

et al bioRxiv.

Insert

MLV Gag (UniProt ID: P03332) (gag MoMLV)

Use

Piggybac

Tags

EIF4E

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-HIS (V5 and HA)

Plasmid

#222487

Purpose

Co-expression of two genes in yeast with V5 and HA tags and HIS3 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Use

Tags

HA and V5

Expression

Yeast

Mutation

Promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Lifeact-LSSmOrange

Plasmid

#218976

Purpose

In vivo visualization of actin cytoskeleton

Depositor

Amro Hamdoun

Insert

Lifeact-LSSmOrange

Use

Tags

LSSmOrange

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-hTimeless (pc4729)

Plasmid

#216861

Purpose

Mammalian expression vector encoding GFP tagged human Timeless

Depositor

Cristina Cardoso

Article

Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

Insert

Timeless (TIMELESS Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-hTipin (pc4732)

Plasmid

#216862

Purpose

Mammalian expression vector encoding GFP tagged human Tipin

Depositor

Cristina Cardoso

Article

Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

Insert

Tipin (TIPIN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TMPRSS3

Plasmid

#221298

Purpose

Expression of TMPRSS3 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TMPRSS3 (pLxIS) (Tmprss3 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS2

Plasmid

#218712

Purpose

Expresses N-terminally EGFP-tagged BBS2 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS2 (BBS2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV/hSyn-SCLM

Plasmid

#216760

Purpose

AAV2 transfer vector with the human synapsin I promoter for neuronal expression of SuperClomeleon

Depositor

George Augustine

Insert

SuperClomeleon

Use

AAV

Tags

Expression

Mammalian

Mutation

S30R and 11 AA deletion in the Cerulean CFP moiet…

Promoter

hSyn (human synapsin I)

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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