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PurposeKI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129092 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneHR180-PA
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Vector typehomologous recombination donor plasmid
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP and LGR5 homology arms
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SpeciesH. sapiens (human)
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Entrez GeneLGR5 (a.k.a. FEX, GPR49, GPR67, GRP49, HG38)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Includes 5' and 3' LGR5 homologous arms.
mRuby is a part of the selection marker mRuby T2A puro, allowing researchers to visually check whether KI is successful.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HR180-LGR5-GFP was a gift from Toshiro Sato (Addgene plasmid # 129092 ; http://n2t.net/addgene:129092 ; RRID:Addgene_129092) -
For your References section:
Visualization and targeting of LGR5(+) human colon cancer stem cells. Shimokawa M, Ohta Y, Nishikori S, Matano M, Takano A, Fujii M, Date S, Sugimoto S, Kanai T, Sato T. Nature. 2017 May 11;545(7653):187-192. doi: 10.1038/nature22081. Epub 2017 Mar 29. 10.1038/nature22081 PubMed 28355176