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Showing: 2901 - 2950 of 156570 results

  5. pGL4_CRE-CMVmin-luc2

    Plasmid
    #194384
    Purpose
    Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter
    Depositor
    Michael Wehr
    Article
    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48
    Insert
    6xCRE-CMVmin-luc2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV minimal
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194384-plasmid-map-sequence-id-381615

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  13. pLJM1-EGFP-GRAM-H

    Plasmid
    #211703
    Purpose
    Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (pLJM1-EGFP-GRAM1b G187L)
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Leucine
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211703-plasmid-map-sequence-id-419205

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  16. pCAG-T7pol

    Plasmid
    #59926
    Purpose
    Expression vector for T7 RNA polymerase gene
    Depositor
    Ian Wickersham
    Insert
    T7 RNA polymerase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    59926-plasmid-map-sequence-id-418961

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  17. TFORF0707

    Plasmid
    #143889
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NCOR1 (NCOR1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    143889-plasmid-map-sequence-id-408610

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  22. p663-UBC-PM-TurboID-V5_IDG-K

    Plasmid
    #214455
    Purpose
    Gateway destination clone of PM-TurboID (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    PM-TurboID-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214455-plasmid-map-sequence-id-423213

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  23. mCherry-Rab5a-7

    Plasmid
    #55126
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Rab5a
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55126-plasmid-map-sequence-id-133168

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  24. pET His6 MBP TEV LIC cloning vector (1M)

    Plasmid
    #29656
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    6xHis, MBP, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only
    29656-plasmid-map-sequence-id-237947

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  26. AAV-CamKIIa-jGCaMP8f-WPRE

    Plasmid
    #176750
    Purpose
    AAV-mediated expression of GCaMP8f under the CamKIIa promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV5
    Insert
    jGCaMP8f
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CamKIIalpha
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    176750-plasmid-map-sequence-id-408507

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  29. pCMV-T7 RNA POL

    Plasmid
    #138352
    Purpose
    Expressing T7 RNA Polymerase in mammalian cells
    Depositor
    Baisong Lu
    Insert
    T7 RNA POL
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    138352-plasmid-map-sequence-id-273271

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  31. pAD-LyseR

    Plasmid
    #99244
    Purpose
    For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)
    Depositor
    Jeff Hasty
    Article
    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102
    Insert
    phage lambda gene R (R phage lambda)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    beta-lactamase promoter
    Available since
    Oct. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99244-plasmid-map-sequence-id-191038

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  32. pMH-SFB

    Plasmid
    #99391
    Purpose
    Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)
    Depositor
    Michael Huen
    Article
    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114
    Type
    Empty backbone
    Use
    Tags
    SFB (S protein-FLAG-Streptavidin binding peptide)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    99391-plasmid-map-sequence-id-194202

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  34. pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

    Plasmid
    #212962
    Purpose
    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_non-targeting spacer
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212962-plasmid-map-sequence-id-422485

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  35. pX333

    Plasmid
    #64073
    Purpose
    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.
    Depositor
    Andrea Ventura
    Article
    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    3XFLAG-Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CBh; U6
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64073-plasmid-map-sequence-id-360030

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  36. pBUE411

    Plasmid
    #62200
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62200-plasmid-map-sequence-id-395472

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  44. pcDNA3-mRFP

    Plasmid
    #13032
    Purpose
    Mammalian expression vector for cloning your gene fused to mRFP
    Depositor
    Doug Golenbock
    Has Service
    Cloning Grade DNA
    Insert
    Monomer Red Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
    13032-plasmid-map-sequence-id-211651

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  46. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61357-plasmid-map-sequence-id-358013

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  49. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90085-plasmid-map-sequence-id-182691

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Showing: 2901 - 2950 of 156570 results