pLenti6.3 GCN1-3xFlag_IRES-iRFP
(Plasmid
#198388)
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PurposeGCN1 expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 198388 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLenti6.3 (SV40-bsd removed)
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Vector typeMammalian Expression, Lentiviral
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Selectable markersiRFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCN1
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SpeciesH. sapiens (human)
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Entrez GeneGCN1 (a.k.a. GCN1L, GCN1L1, PRIC295)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFlag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti6.3 GCN1-3xFlag_IRES-iRFP was a gift from Jack Taunton (Addgene plasmid # 198388 ; http://n2t.net/addgene:198388 ; RRID:Addgene_198388) -
For your References section:
An E3 ligase network engages GCN1 to promote the degradation of translation factors on stalled ribosomes. Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J. Cell. 2023 Jan 19;186(2):346-362.e17. doi: 10.1016/j.cell.2022.12.025. Epub 2023 Jan 12. 10.1016/j.cell.2022.12.025 PubMed 36638793
