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Showing: 1161 - 1180 of 52769 results

  4. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid
    #58477
    Purpose
    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Article
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed Arginine 62 to Aspartic Acid
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    58477-plasmid-map-sequence-id-90386

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  6. Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

    Plasmid
    #58492
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    IRE1a (ERN1 Human)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    deleted amino acids P29 to D408 and bearing mutat…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    58492-plasmid-map-sequence-id-90361

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  14. pCAGGS-CD4-YEML-myc

    Plasmid
    #58538
    Purpose
    Expresses human CD4 with the IFITM3 endocytosis motif (YEML) and a C-terminal myc tag in mammalian cells.
    Depositor
    Jacob Yount
    Article
    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9
    Insert
    CD4 (CD4 Human)
    Use
    Tags
    IFITM3 endocytosis Tyrosine-Glutamate-Methionine-…
    Expression
    Mammalian
    Mutation
    Added C-terminal Tyrosine-Glutamate-Methionine-Le…
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  15. HSC1-HS4-D4Z4c-GiP

    Plasmid
    #58541
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1-alpha
    Availability
    Academic Institutions and Nonprofits only
    58541-plasmid-map-sequence-id-90716

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Showing: 1161 - 1180 of 52769 results