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Plasmid#196369Purposeprotein expression of the GST-tagged Cereblon Thalidomide binding domainDepositorInsertCRBN (CRBN Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationcontains only T319-T425PromoterT7Available sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE
Plasmid#207664PurposeNeuropeptide degradationDepositorInsertNEP (MME Human)
UseAAV and Cre/LoxTagsmRUBY3ExpressionMutationAddition of signaling peptide (POMC) on the N-ter…PromoterhSynapsinAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4
Plasmid#193294Purposeepisomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversionDepositorUseTagsExpressionMammalianMutationSOX2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CDKN1A_WT
Plasmid#82201PurposeGateway Donor vector containing CDKN1A , part of the Target Accelerator Plasmid Collection.DepositorInsertCDKN1A (CDKN1A Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_HA1h
Plasmid#190473PurposeExpress the high-affinity histamine sensor GRAB_HA1h in neuronsDepositorInsertHistamine sensor GRAB_HA1h (high affinity)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC7A5_STOP
Plasmid#161363PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC7A5 (SLC7A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEGFP-HRas G12V
Plasmid#18666DepositorInsertH-Ras (HRAS Human)
UseTagsmonomeric EGFPExpressionMammalianMutationG12V mutation in HRasPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2
Plasmid#223164Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-BI-AR
Plasmid#171234PurposeMammalian expression of 2xHA-tagged human androgen receptor (hAR)DepositorInsert2xHA-tagged human androgen receptor (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab7A T22N
Plasmid#28048DepositorInsertRab7A (RAB7A Human)
UseTags2xHA and EGFPExpressionMammalianMutationT22NPromoterAvailable sinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-Cul7
Plasmid#20695DepositorInsertcullin 7 (CUL7 Human)
UseTagsMyc3ExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAR015 Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD
Plasmid#195544PurposedCas12a effector fusion for manipulating transcriptionDepositorArticleInsertLenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD
UseCRISPR and LentiviralTags3XHAExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti MS2-P65-HSF1_Hygro
Plasmid#61426Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2DepositorInsertMS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Sec24D
Plasmid#32677DepositorInsertSec24D (SEC24D Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_eCB2.0
Plasmid#164606PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neuronsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVTagsExpressionMutationS383TPromoterhSynAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hDAT
Plasmid#32810DepositorInsertDAT (SLC6A3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ATR Kinase dead
Plasmid#31612DepositorInsertATR (ATR Human)
UseTagsFlagExpressionMammalianMutationK2327R (kinase dead)PromoterAvailable sinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOPTN-EGFP
Plasmid#27052DepositorInsertoptineurin (OPTN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab8a-wt
Plasmid#86075PurposeA mammalian expression plasmid encoding Rab8a wild type with N-terminus EGFP.DepositorInserthRab8A (RAB8A Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only