pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE
(Plasmid
#207664)
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PurposeNeuropeptide degradation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207664 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3
- Backbone size w/o insert (bp) 5116
- Total vector size (bp) 8069
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Vector typeAAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNEP
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Alt nameNeprilysin
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SpeciesH. sapiens (human)
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Insert Size (bp)2953
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MutationAddition of signaling peptide (POMC) on the N-terminus
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GenBank IDNP_000893.2
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Entrez GeneMME (a.k.a. CALLA, CD10, CMT2T, NEP, SCA43, SFE)
- Promoter hSynapsin
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Tag
/ Fusion Protein
- mRUBY3 (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer ACTTGCAAATGGGCTTGCCACGCACTTC
- 3′ sequencing primer CTAGAATTCGGTACCGCTAGCCCCCACCATGCCGAGATC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE was a gift from Sung Han (Addgene plasmid # 207664 ; http://n2t.net/addgene:207664 ; RRID:Addgene_207664)