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Plasmid#12436DepositorInsertbZIP of C/EBPa (CEBPA Human)
UseTagsGSTExpressionMammalianMutationpEBG vector is from Bruce Mayer, may be different…PromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
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GANLS-DEVD-BNES
Plasmid#50835PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddGFP A and ddRFP B
UseTagsNES sequence LALKLAGLDIGS and triplicated NLS seq…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLC-RFP657-CASP8
Plasmid#75164PurposeLentiCRISPR-RFP657 with sgRNA targeting human Caspase-8DepositorInsertCASP8 sgRNA (CASP8 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DHX36iso2E335A
Plasmid#159588PurposeExpress N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cellsDepositorInsertDHX36 (DHX36 Human)
UseTagsFLAG HAExpressionMammalianMutationE335APromoterCMVAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
TMED3-hLOV-TEVcs-GAL4bd-VP64-V5
Plasmid#170996PurposeHiLITR transcription factor with full-length TMED3 targeting sequence (ER/Golgi)DepositorInsertTMED3(FL)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (TMED3 Synthetic)
UseLentiviral and Synthetic BiologyTagsV5ExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKK-NoTag
Plasmid#105767PurposeControl vector for pKK series (encodes only SLIC arms (TEV-L and TEV-R)); expression without tag. Useful to design other vectors; any tag can be added.DepositorTypeEmpty backboneUseFlp-in competentTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-IKZF2-V2-3xHA-pGK-Pur
Plasmid#69052Purposelentiviral expression of IKZF2 variant 2 with HA tagDepositorInsertIKZF2 (IKZF2 Human)
UseLentiviralTagsHAExpressionMammalianMutationsplice variant 2PromoterUBCAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
yHS1-M7A,H102A
Plasmid#159152PurposeCytosolic labile heme reporterDepositorInsertyHS1-M7A,H102A
UseTagsExpressionYeastMutationMutated both Met 7 and His 102 of the cyt b562 mo…PromoterGPDAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(K363TAG)-FLAG
Plasmid#182656PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K363 & C-terminal FLAG tag (DYKDDDDK) in mammalian cells. Can be used for amber codon suppression & click labeling of NFL.DepositorInsertmouse neurofilament light chain with a K363TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK363TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOUPc-UL148-HA
Plasmid#179343PurposeAll-in-one "tet-on" 3G lentiviral vector plasmid encoding human cytomegalovirus UL148 ER resident glycoprotein with a C-terminal HA tag fused to its cytoplasmic tailDepositorInserthuman cytomegalovirus UL148 fused to HA epitope
UseLentiviral and Synthetic Biology; Hiv-1 based rep…TagsHA tagExpressionMammalianMutationinsert matches UL148 from strain TB40/EPromoterTet responsive element 3GAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode
Plasmid#170217PurposeBarcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCASP8 (CASP8 Human)
UseLentiviralTagsV5ExpressionMutationC360APromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Egr3-EYFP-WPRE
Plasmid#186417PurposeOverexpression of Egr3 transcript variant 1 CDS under EF-1α promoterDepositorInsertEarly growth response 3 (Egr3 Mouse)
UseAAVTagsEYFPExpressionMutationPromoterEF-1-alphaAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-218_HNF4A2
Plasmid#31090DepositorInsertHNF4A (HNF4A Human)
UseFlp/frtTagsmycExpressionMammalianMutationsplice variant 2 of HNF4A, deletion of CMV promo…PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc-oDam_f_GFPoNLS
Plasmid#85818PurposeiDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc oDam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-138_HNF4A8
Plasmid#31093DepositorInsertHNF4A (HNF4A Human)
UseFlp/frtTagsmycExpressionMammalianMutationsplice variant 8 of HNF4A, deletion of CMV promo…PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL3-FLIP pr4
Plasmid#19129DepositorInsertFLIP promoter (CFLAR Human)
UseLuciferaseTagsExpressionMammalianMutationThe sequence corresponds to positions -503 to +10…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
CMV-68_HNF4A2
Plasmid#31092DepositorInsertHNF4A (HNF4A Human)
UseFlp/frtTagsmycExpressionMammalianMutationsplice variant 2 of HNF4A, deletion of CMV promo…PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS
Plasmid#59388PurposeDestination vector with RBPMS for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-138_HNF4A2
Plasmid#31091DepositorInsertHNF4A (HNF4A Human)
UseFlp/frtTagsmycExpressionMammalianMutationsplice variant 2 of HNF4A, deletion of CMV promo…PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only