pCMV_NFL(K363TAG)-FLAG
(Plasmid
#182656)
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PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K363 & C-terminal FLAG tag (DYKDDDDK) in mammalian cells. Can be used for amber codon suppression & click labeling of NFL.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182656 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemouse neurofilament light chain with a K363TAG mutation
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Alt nameNFL(K363TAG)
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SpeciesM. musculus (mouse)
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MutationK363TAG
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Entrez GeneNefl (a.k.a. CMT2E, NF-L, NF68, Nfl)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG tag (DYKDDDDK) (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMVfw
- 3′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV_NFL(K363TAG)-FLAG was a gift from Ivana Nikić-Spiegel (Addgene plasmid # 182656 ; http://n2t.net/addgene:182656 ; RRID:Addgene_182656) -
For your References section:
Minimal genetically encoded tags for fluorescent protein labeling in living neurons. Arsic A, Hagemann C, Stajkovic N, Schubert T, Nikic-Spiegel I. Nat Commun. 2022 Jan 14;13(1):314. doi: 10.1038/s41467-022-27956-y. 10.1038/s41467-022-27956-y PubMed 35031604