P2_HNF4A8
(Plasmid
#31094)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31094 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5/FRT/TO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5150
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Vector typeMammalian Expression ; Flp/FRT
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHNF4A
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SpeciesH. sapiens (human)
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Insert Size (bp)1350
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Mutationsplice variant 8 derived from promoter P2
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GenBank IDNM_175914
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Entrez GeneHNF4A (a.k.a. FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF-14, TCF14)
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Tag
/ Fusion Protein
- myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
for Flp mediated site directed integration, tetracycline inducibilty by Tet operator in P2 promoter of HNF4A
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
P2_HNF4A8 was a gift from Gerhart Ryffel (Addgene plasmid # 31094 ; http://n2t.net/addgene:31094 ; RRID:Addgene_31094) -
For your References section:
Improved conditional expression systems resulting in physiological level of HNF4alpha expression confirm HNF4alpha induced apoptosis in the pancreatic beta-cell line INS-1. Senkel S, Waldner C, Ryffel GU, Thomas H. BMC Res Notes. 2009 . 2():210. 10.1186/1756-0500-2-210 PubMed 19835622