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Plasmid#25933DepositorInsertRapR-Src (Src Mouse)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
Fragment1 with TLS2
Plasmid#196983PurposeGateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.DepositorInsertIntermediate fragment for assembly of gRNA-TLS2 construct
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-SPARC2-I-LexA::p65
Plasmid#133563PurposeSwap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locusDepositorInsertLexA::p65
UseCRISPRTagsExpressionInsectMutationPromoter20X UASAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.GCaMP6f.WPRE.SV40
Plasmid#100833PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW-LIC-N8C_Fab3b
Plasmid#203685PurposeExpresses N8C_Fab3bDepositorInsertN8C_Fab3b
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Rh
Plasmid#61649PurposeGateway vector for generating red fluorescent protein fusions in fungiDepositorInsertmonomeric red fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
CoV2-N-WT-Hu1
Plasmid#177937PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.DepositorInsertSARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN1
Plasmid#180282PurposeCas9 cassette (TU1)DepositorInsertConLS - pPGK1 - Cas9 - tPGK1 - ConR1
UseTagsExpressionBacterialMutationPromoterpPGK1Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
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AAV-Ef1a-InteinC-CreC
Plasmid#187615PurposeAAV vector encoding C-terminal portion of split-Intein-mediated split-CreDepositorInsertInteinC-CreC
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2-N1
Plasmid#54525PurposeLocalization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580DepositorTypeEmpty backboneUseTagsmEos3.2ExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENN.AAV.cTNT.PI.eGFP.WPRE.rBG
Plasmid#105543PurposeAAV expression of EGFP from cTNT promoterDepositorHas ServiceAAV9InsertcTNT-PI-EGFP
UseAAVTagsExpressionMammalianMutationPromotercTNTAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS426 Gal TDP43 GFP
Plasmid#27467DepositorInsertTDP-43 (TARDBP Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462)
Plasmid#48141PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62987DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MRLC T18A,S19A
Plasmid#35681DepositorInsertMRLC1 (MYL9 Human)
UseTagsEGFPExpressionMammalianMutationT18A and S19APromoterCMVAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA
Plasmid#51095PurposeCre dependent expression of ChIEF kinetic variant E162A/T198C with physically uncoupled dTomato fluorophoreDepositorInsertChIEF(E162A/T198C)
UseAAVTagsP2A-dTomatoExpressionMammalianMutationE162A/T198CPromoterEF1aAvailable sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-KASH2
Plasmid#187017PurposeInducible disruption of LINC ComplexDepositorInsertKASH2
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon TVA-mCherry
Plasmid#131779PurposeINTRSECT 2.0DepositorInsertTVA-mCherry
UseAAV; IntrsectTagsmCherryExpressionMammalianMutationPromoternEFAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe-SH1446
Plasmid#112507PurposeExpresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.DepositorInsertPOT family transporter
UseTagsC terminal TEV GFP octa-His affinity tagExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RSV-SpCas9
Plasmid#85450PurposeExpress SpCas9 in mammalian cellsDepositorInsertpRSV
UseAAVTagsExpressionMutationPromoterpRSVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only