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Plasmid#205007PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between.DepositorInsertRvSAHS1-mEGFP with 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag
Plasmid#209652PurposeExpression of iATPSnFR2, medium affinityDepositorInsertiATPSnFR2.A95K.HaloTag
UseAAVTagsExpressionMutationA95KPromoterCAGAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB076i3
Plasmid#79198PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-N1
Plasmid#54595PurposeLocalization: N1 Cloning Vector, Excitation: 383, Emission: 448DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsEBFP2ExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLH-sgRNA-Sirius-8XMS2
Plasmid#121939PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-EF1α-EGFP
Plasmid#21320PurposeControl Lentiviral vector with strong EF1a promoter, expresses EGFPDepositorInsertEnhanced Green Fluorescence Protein
UseLentiviralTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR.v2.TetON_Cas13d_U6_DR
Plasmid#196727PurposeSortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.DepositorInsertRfxCas13d-T2A-miRFP670nano
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterTREAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
VN-Tau (wt)
Plasmid#87368Purposeexpresses hTau fused to the N-terminal part of VenusDepositorInsertMAPT (MAPT Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGIP
Plasmid#26777PurposeLentiviral vector for stable integration of GFP expression cassette with puromycin selection.DepositorInsertEF1a-GFP-IRES-Puro (gfp )
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pY108 (lenti-AsCpf1)
Plasmid#84739PurposeLenti virus delivery of AsCpf1 and crRNA guideDepositorInsertshuAsCpf1
puromycin resistance gene
UseLentiviralTags3xHA, NLS, and P2AExpressionMutationPromoterEFSAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP1
Plasmid#79873PurposeBacillus subtilis dCas9 expression vector; integrates into lacA/ganADepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationD10A, H840APromoterxylAAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
REEP5-EGFP
Plasmid#210363PurposeExpresses REEP5 fused with EGFP in mammalian cellsDepositorInsertREEP5 (REEP5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9-T2A-GFP
Plasmid#53191PurposeCo-expresses human optimized S. pyogenes dCas9 and GFPDepositorInserthumanized dead Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterhUbCAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
nAChR alpha4 YFP
Plasmid#15245DepositorInsertalpha4-YFP-M (Chrna4 Mouse)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCINeoFlag ALIX
Plasmid#89859PurposeExpresses full length Flag-tagged ALIX in mammalian cells; internal ID WISP03-308DepositorInsertALIX (PDCD6IP Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-T7-EcKlenow (LM2692)
Plasmid#208963PurposeUnfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.DepositorInsertEcKlenow-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationEcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGW-GOI-TurboID
Plasmid#209378PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-FER
Plasmid#23921DepositorInsertFER (FER Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
iRFP-C1
Plasmid#54786PurposeLocalization: C1 Cloning Vector, Excitation: 690, Emission: 713DepositorTypeEmpty backboneUseTagsiRFPExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-EGFP-Cdc42-Q61L
Plasmid#12986DepositorInsertCdc42 constitutively active (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationQ61L Constitutively activePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only